Recombinant Human PKC epsilon/PKCE Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q02156
| 别称 |
Protein Kinase C Epsilon Type;nPKC-Epsilon;PRKCE;PKCE
|
| 表达系统 |
E.coli
|
| 序列 |
Gln580-Pro737
|
| 蛋白编码 |
Q02156
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| 种属 |
Human
|
| 计算分子量 |
19.6 kDa
|
| 表观分子量 |
20 kDa
|
| 标签 |
C-His
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| 纯度 |
> 90 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain; one C2 domain; one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions; such as neuron channel activation; apoptosis; cardioprotection from ischemia; heat shock response; as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters; a class of tumor promoters.
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实验操作视频
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