Recombinant Human Rock-2 protein (His tag)
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- 表达系统： E.coli
- 蛋白编码： O75116
| 别称 |
Rho kinase 2;Rho-associated;Rho-associated protein kinase 2;ROCK 2;Rock II;Rock2;ROCK2;Rock2m;ROK alpha;ROKalpha
|
| 表达系统 |
E.coli
|
| 序列 |
Leu 904-Gly 1140
|
| 蛋白编码 |
O75116
|
| 种属 |
Human
|
| 计算分子量 |
26.0 kDa
|
| 表观分子量 |
35 kDa
|
| 标签 |
N-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
Please contact us for more information.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
ROCK2/II (Rho-associated protein kinase 2; also Rho-kinase alpha ) is a member of the AGC serine/threonine protein kinase family. ROCK2 is a downstream target of activated RhoA. Its activation results in myosin phosphorylation with subsequent muscle contraction. Human ROCK2 is 1388 amino acids (aa) in length, and appears to be present as a dimer. It contains a protein kinase domain (aa 92?354), a coiled-coil region (aa 429?1131) and a zinc-finger region (aa 1260?1315). Two isoforms show an alternate start site at M686, and a three aa substitution for the N-terminal 444 amino acids.
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实验操作视频
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