Recombinant Human SMAD4 Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： Q13485
| 别称 |
Mothers Against Decapentaplegic Homolog 4;MAD Homolog 4;Mothers Against DPP Homolog 4;Deletion Target in Pancreatic Carcinoma 4;SMAD Family Member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4;MADH4
|
| 表达系统 |
E.coli
|
| 序列 |
Met 1-Asp552
|
| 蛋白编码 |
Q13485
|
| 种属 |
Human
|
| 计算分子量 |
61.5 kDa
|
| 表观分子量 |
62 kDa
|
| 标签 |
C-His
|
| 纯度 |
> 80 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|
| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
|
| 制剂 |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
|
| 背景 |
SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
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实验操作视频
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