RIPK1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Hela,HepG2 Verified Samples in IHC:Human liver cancer |
| Dilution | WB 1:500-1:2000;IHC 1:50-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of human RIPK1 |
| Abbre | RIPK1 |
| Synonyms | Cell death protein RIP;FLJ39204;OTTHUMP00000039163;Receptor (TNFRSF) interacting serine threonine kinase 1;receptor interacting protein 1;Receptor interacting protein;Receptor interacting protein kinase 1;Receptor interacting serine threonine protein kinase 1;Receptor TNFRSF interacting serine threonine kinase 1;Receptor-interacting protein 1;Receptor-interacting serine/threonine-protein kinase 1;Rinp;RIP 1;RIP;Rip-1;RIP1;RIPK 1;Ripk1;RIPK1;Serine threonine protein kinase RIP;Serine/threonine-protein kinase RIP抗体 |
| Swissprot | |
| Calculated MW | 76 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. |
| Concentration | 0.7 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Cell Biology; Signal transduction |
| Conjugation | Unconjugated |
| Background | Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex. |
Western blot analysis of Hela and HEPG2 cell lysates using RIPK1 Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using RIPK1 Polyclonal Antibody at dilution of 1:85(×200)
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
