ROCK1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主： Rabbit
- 反应性： Mouse;Rat
- 应用性： IHC
| Verified Samples |
Verified Samples in IHC:Mouse kidney,Rat spleen |
| Dilution | IHC 1:100-1:400 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Rock1 |
| Abbre | ROCK1 |
| Synonyms | coiled-coil-containing protein kinase 1;coiled-coil-containing protein kinase I;MGC131603;MGC43611;p160 Rhoassociated coiled coil-forming protein kinase;p160 ROCK-1;p160 ROCK1;p160ROCK;PRO0435;Renal carcinoma antigen NY REN 35;Renal carcinoma antigen NY-REN-35;Rho associated coiled coil containing protein kinase 1;Rho associated protein kinase 1;Rho kinase;Rho-alpha kinase;Rho-associated;Rho-associated protein kinase 1;ROCK I;ROCK-I;ROCK1;ROCK1;Rok;rokalpha抗体 |
| Swissprot | |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes. |
| Concentration | 300 μg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Cell Biology; Signal Transduction |
| Conjugation | Unconjugated |
| Background | This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. |
Immunohistochemistry analysis of paraffin-embedded mouse kidney using ROCK1 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded rat spleen using ROCK1 Polyclonal Antibody at dilution of 1:300.
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
