SMAD7 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse; Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Mouse heart Verified Samples in IHC:Human esophagus cancer,Human tonsil,Rat kidney |
| Dilution | WB 1:500-1:2000;IHC 1:40-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide of human SMAD7 |
| Abbre | SMAD7 |
| Synonyms | CRCS3;FLJ16482;hSMAD 7;hSMAD7;MAD (mothers against decapentaplegic Drosophila) homolog 7;MAD;Mad homolog 7;MAD homolog 8;MAD mothers against decapentaplegic homolog 7;MADH 7;MADH 8;MADH6;MADH8;Mothers Against Decapentaplegic Drosophila Homolog of 6;Mothers Against Decapentaplegic Drosophila Homolog of 7;Mothers against decapentaplegic homolog 7;Mothers against decapentaplegic homolog 8;Mothers against DPP homolog 7;Mothers against DPP homolog 8;SMA- AND MAD-RELATED PROTEIN 7;SMAD 7;SMAD;SMAD family member 7;SMAD;mothers against DPP homolog 7 (Drosophila);SMAD;mothers against DPP homolog 7;SMAD6;Smad7;SMAD7抗体 |
| Swissprot | |
| Calculated MW | 46 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). |
| Concentration | 1.14 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Signal Transduction |
| Conjugation | Unconjugated |
| Background | SMAD7,also named as Mothers against decapentaplegic homolog 7,is a 426 amino acid protein,which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1,which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
Western blot analysis of Mouse heart tissue lysate using SMAD7 Polyclonal Antibody at dilution of 1:900
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SMAD7 Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human tonsil tissue using SMAD7 Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Rat kidney using SMAD7 Polyclonal Antibody at dilution of 1:50
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| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
