本公司产品仅供体外研究使用,不用于临床诊断

Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

总胆固醇(TC)比色法测试盒(单试剂COD-PAP法)

规格: / 48T(44 samples) / 96T(92 samples)
价格: / ¥200 / ¥320

货号:E-BC-K109-M

检测仪器及波长: 酶标仪(495-525 nm)

样本类型: 血清(浆);组织

  • 详情
  • 文献(36)
  • 检测原理

    总胆固醇(Total Cholesterol,TC)包括游离胆固醇和胆固醇酯。胆固醇酯可被胆固醇酯酶(cholesterol esterase,CE)水解成胆固醇和游离脂肪酸,胆固醇在胆固醇氧化酶(cholesterol oxidase,CO)的氧化作用下生成△4-胆甾烯酮和过氧化氢。过氧化氢在4-氨基安替吡啉和酚存在时,经过氧化物酶(peroxidase,POD)催化,反应生成苯醌亚胺非那腙的红色醌类化合物,其颜色深浅与TC含量成正比。

    关键信息

    指标简称 TC
    样本类型 血清(浆);组织
    灵敏度 0.29 mmol/L
    检测范围 0.29-25.85 mmol/L
    精密度 批间差:8.3%批内差:3.1%
    检测仪器及波长 酶标仪
    自备试剂 无水乙醇
    保存条件 2-8℃
    有效期 6个月

    样本稀释倍数

    在正式检测前,需选择2-3个预期差异大的样本稀释成不同浓度进行预实验,结合本试剂盒的检测范围(0.29-25.85 mmol/L),选取最佳稀释倍数,进行正式批量实验。不同样本的稀释比例范围如下表(仅供参考):

    样本

    稀释倍数

    样本

    稀释倍数

    人血清

    不稀释

    10%小鼠肝匀浆

    不稀释

    小鼠血清

    不稀释

    10%小鼠肾匀浆

    不稀释

    大鼠血浆

    不稀释

    10%小鼠心匀浆

    不稀释

    HepG2 细胞

    不稀释

    注:血清(浆)样本的稀释液为生理盐水(0.9% NaCl)或PBS0.01MpH 7.4),动物组织与细胞样本的稀释液为异丙醇。

    客户发表文献

    1. 文献: Phosphomevalonate Kinase Controls β-Catenin Signaling via the Metabolite 5-Diphosphomevalonate
      样品类型: Serum
    2. 文献: Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal
      样品类型: liver
    3. 文献: Total Flavonoids of Drynariae Rhizoma Improve Glucocorticoid-Induced Osteoporosis of Rats: UHPLC-MS-Based Qualitative Analysis, Network Pharmacology Strategy and Pharmacodynamic Validation
      样品类型: Serum
    4. 文献: Analysis of the mechanism of action of quercetin in the treatment of hyperlipidemia based on metabolomics and intestinal flora
      样品类型: serum
    5. 文献: Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms
      样品类型: serum
    6. 文献: N6-Methyladenosine Methyltransferase METTL3 Promotes Angiogenesis and Atherosclerosis by Upregulating the JAK2/STAT3 Pathway via m6A Reader IGF2BP1.
      样品类型: plasma
    7. 文献: Ascophyllum nodosum and Fucus vesiculosus Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet–Induced Hyperlipidemia Rats
      样品类型: liver
    8. 文献: TRIM64 promotes ox-LDL-induced foam cell formation, pyroptosis, and inflammation in THP-1-derived macrophages by activating a feedback loop with NF-κB via IκBα ubiquitination
      样品类型: THP-1 cells
    9. 文献: Integrating metabolomics and network pharmacology to investigate Panax japonicus prevents kidney injury in HFD/STZ-induced diabetic mice
      样品类型: serum
    10. 文献: AFM detects the effects of acidic condition on the size and biomechanical properties of native/oxidized low-density lipoprotein
      样品类型: plasma
    11. 文献: Gymnaster Koraiensis Extract Alleviated Metabolic Syndrome Symptoms and Stimulated UCP1‐Independent Energy Consumption via AMPK Activation in White Adipose Tissue
      样品类型: Serum
    12. 文献: Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice
      样品类型: serum,liver
    13. 文献: Metabolic Consequences of Neuronal HIF1α-Deficiency in Mediobasal Hypothalamus in Mice
      样品类型: serum
    14. 文献: Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota
      样品类型: liver,fecal,serum
    15. 文献: Paraoxonase 1 Ameliorates Renal Lipotoxicity by Activating Lipophagy and Inhibiting Pyroptosis
      样品类型: plasma
    16. 文献: Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion
      样品类型: serum
    17. 文献: Zexie Tang targeting FKBP38/mTOR/SREBPs pathway improves hyperlipidemia
      样品类型: serum
    18. 文献: Alpha-lipoic acid attenuates silica-induced pulmonary fibrosis by improving mitochondrial function via AMPK/PGC1α pathway activation in C57BL/6J mice
      样品类型: serum
    19. 文献: Heracleum dissectum Ledeb. ethanol extract attenuates metabolic syndrome symptoms in high-fat diet-induced obese mice by activating adiponectin/AMPK signaling
      样品类型: blood
    20. 文献: Isoliquiritigenin ameliorates caerulein‐induced chronic pancreatitis by inhibiting the activation of PSCs and pancreatic infiltration of macrophages
      样品类型: Serum
    21. 文献: Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression
      样品类型: serum
    22. 文献: Serum Levels of Free Fatty Acids in Obese Mice and Their Associations with Routine Lipid Profiles
      样品类型: serum
    23. 文献: Coating beef tallow with calcium soap improves dietary pellet quality and promotes an effective diet-induced obese mouse model
      样品类型: plasma
    24. 文献: Effects of naturally-produced lovastatin on carcass characteristics, muscle physico-chemical properties and lipid oxidation and cholesterol content in goats
      样品类型: serum
    25. 文献: Quillaja saponaria and/or Yucca schidigera ameliorate water quality, growth performance, blood health, intestine and gills histomorphology of Nile tilapia, Oreochromis niloticus
      样品类型: serum
    26. 文献: Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model
      样品类型: Serum
    27. 文献: Dehydroabietic acid improves nonalcoholic fatty liver disease through activating the Keap1/Nrf2-ARE signaling pathway to reduce ferroptosis
      样品类型: Tissue homogenate(liver)
    28. 文献: Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats
      样品类型: plasma
    29. 文献: Dietary sweet cherry anthocyanins attenuates diet-induced hepatic steatosis by improving hepatic lipid metabolism in mice
      样品类型: liver tissue
    30. 文献: Pitavastatin attenuates atherosclerosis by suppressing NF-κB signaling in a high-cholesterol diet plus balloon catheter injury rabbit model
      样品类型: serum
    31. 文献: Toxic effects of subchronic oral acetamiprid exposure in rats:
      样品类型: plasma
    32. 文献: Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:
      样品类型: Plasma
    33. 文献: Tea polyphenols attenuate liver inflammation by modulating obesity-related genes and down-regulating COX-2 and iNOS expression in high fat-fed dogs
      样品类型: Serum,Tissue homogenate
    34. 文献: Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice
      样品类型: Serum
    35. 文献: Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats
      样品类型: serum
    36. 文献: IMPACTS OF AEROBIC EXERCISE ON THE OBESITY OF ADOLESCENTS AND THEIR LIPID METABOLISM
      样品类型: serum
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