Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
总谷胱甘肽(T-GSH)/氧化型谷胱甘肽(GSSG)比色法测试盒
规格: | / 96T(80 samples) |
价格: | / ¥580 |
货号:E-BC-K097-M
检测仪器及波长: 酶标仪(405-415 nm,最适检测波长412 nm)
样本类型: 血清(浆);动物组织;红细胞和培养细胞中的总谷胱甘肽(T-GSH)和氧化型谷胱甘肽(GSSG)
检测原理
通过谷胱甘肽还原酶把GSSG还原成GSH,而GSH可以和生色底物DTNB反应产生GSSG和黄色的TNB,总谷胱甘肽(GSSG+GSH)的量决定了黄色的TNB形成量。从而通过测定A412就可以计算出总谷胱甘肽的量。再用适当试剂先清除样品中的GSH然后利用上述反应原理就可以测定出GSSG的含量。
关键信息
指标简称 | T-GSH,GSSG |
样本类型 | 血清(浆);动物组织;红细胞和培养细胞中的总谷胱甘肽(T-GSH)和氧化型谷胱甘肽(GSSG) |
灵敏度 | 0.36 μmol/L |
检测范围 | 0.36-30 μmol/L |
精密度 | 批间差:3.9%批内差:0.6% |
检测仪器及波长 | 酶标仪 |
自备试剂 | 无水乙醇 |
保存条件 | -20℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3个预期差异大的样本稀释成不同浓度进行预实验,不同样本稀释比例如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
10%大鼠心脏匀浆 |
10-20 |
人血浆 |
不稀释 |
10%大鼠肝脏匀浆 |
10-20 |
10%大鼠肾匀浆 |
不稀释 |
10%小鼠脑匀浆 |
2-5 |
HepG2细胞 |
不稀释 |
注:稀释液为试剂三。
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