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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

总超氧化物歧化酶(T-SOD)比色法测试盒(WST-1 法)

规格: / 48T(44 samples) / 96T(92 samples)
价格: / ¥380 / ¥680

货号:E-BC-K020-M

检测仪器及波长: 酶标仪(440-460 nm)

样本类型: 血清;血浆;胸水;腹水;尿液;细胞;各种动植物组织

  • 详情
  • 文献(120)
  • 检测原理

    WST-1法的原理参考下图,WST-1可以和黄嘌呤氧化酶(Xanthine Oxidase,XO)催化产生的超氧化物阴离子(O2.-)反应产生水溶性的甲臜染料(formazan dye),由于SOD能催化超氧化物阴离子发生歧化反应,所以该反应步骤可以被SOD所抑制,因此SOD的活性与甲臜染料的生成量呈负相关,通过对WST-1产物的比色分析即可计算SOD的活力。

    关键信息

    指标简称 T-SOD
    样本类型 血清;血浆;胸水;腹水;尿液;细胞;各种动植物组织
    灵敏度 0.2 U/mL
    检测范围 0.2 -14.4 U/mL
    精密度 批间差:3.7%批内差:2.9%
    检测仪器及波长 酶标仪
    保存条件 Reagent 3 -20℃,其余2-8℃
    有效期 12个月

    样本稀释倍数

    本试剂盒可检测SOD抑制率范围在25%-65%之间,最佳抑制率范围40%-60%,在正式检测前,需选择差异较大的2-3个样本稀释成不同浓度进行预实验,选取在最佳抑制率范围之间的稀释倍数,进行正式批量实验。不同样本稀释比例范围如下表(仅供参考):

    样本

    稀释倍数

    样本

    稀释倍数

    人血清

    3-5

    10%大鼠心匀浆

    80-100

    大鼠血清

    20-30

    HepG2细胞匀浆(3 mgprot/mL

    30-40

    尿液

    不稀释

    10%植物组织匀浆

    5-10

    人胸水

    2

    10%大鼠肾匀浆

    100-120

    细胞上清

    2-3

    10%大鼠脑匀浆

    50-100

    10%大鼠肝匀浆

    340-370

     

     

    :稀释液为生理盐水或PBS(0.01 M,pH 7.4)。

    若SOD抑制率大于65%,需将样本稀释或减少取样量后再测试;若SOD抑制率小于25 %,需增加取样量后再测试。

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