Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
总超氧化物歧化酶(T-SOD)比色法测试盒(WST-1 法)
规格: | / 48T(44 samples) / 96T(92 samples) |
价格: | / ¥380 / ¥680 |
货号:E-BC-K020-M
检测仪器及波长: 酶标仪(440-460 nm)
样本类型: 血清;血浆;胸水;腹水;尿液;细胞;各种动植物组织
检测原理
关键信息
指标简称 | T-SOD |
样本类型 | 血清;血浆;胸水;腹水;尿液;细胞;各种动植物组织 |
灵敏度 | 0.2 U/mL |
检测范围 | 0.2 -14.4 U/mL |
精密度 | 批间差:3.7%批内差:2.9% |
检测仪器及波长 | 酶标仪 |
保存条件 | Reagent 3 -20℃,其余2-8℃ |
有效期 | 12个月 |
样本稀释倍数
本试剂盒可检测SOD抑制率范围在25%-65%之间,最佳抑制率范围40%-60%,在正式检测前,需选择差异较大的2-3个样本稀释成不同浓度进行预实验,选取在最佳抑制率范围之间的稀释倍数,进行正式批量实验。不同样本稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
3-5 |
10%大鼠心匀浆 |
80-100 |
大鼠血清 |
20-30 |
HepG2细胞匀浆(3 mgprot/mL) |
30-40 |
尿液 |
不稀释 |
10%植物组织匀浆 |
5-10 |
人胸水 |
2 |
10%大鼠肾匀浆 |
100-120 |
细胞上清 |
2-3 |
10%大鼠脑匀浆 |
50-100 |
10%大鼠肝匀浆 |
340-370 |
|
|
注:稀释液为生理盐水或PBS(0.01 M,pH 7.4)。
若SOD抑制率大于65%,需将样本稀释或减少取样量后再测试;若SOD抑制率小于25 %,需增加取样量后再测试。
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