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TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

TUNEL原位细胞凋亡检测试剂盒(HRP-DAB显色法)

规格: / 20Assays / 50Assays / 100Assays
价格: / ¥1650 / ¥2850 / ¥4620

货号:E-CK-A331

  • 详情
  • 文献(13)
  • 检测原理

    细胞在发生凋亡时,会激活一些DNA内切酶,剪断核小体间的基因组DNA,暴露的3’-OH可在末端脱氧核酸转移酶(TdT)的催化下加上生物素标记的dUTP,辣根过氧化物酶(HRP)标记的Streptavidin (Streptavidin-HRP)可与之结合,在HRP的催化下通过DAB显色可观测到凋亡细胞,从而可通过普通光学显微镜检测到细胞的凋亡。由于正常的或正在增殖的细胞中几乎没有DNA的断裂,因而没有3'-OH形成,很少能够被标记。这就是TUNEL(TdT-mediated dUTP Nick-End Labeling)法检测细胞凋亡的原理。

    基本信息

    应用类型 细胞凋亡-DNA片段化
    检测方法 DAB显色
    样本类型 石蜡切片,冰冻切片,细胞爬片,细胞涂片
    检测时间 3 hours
    检测仪器 普通光学显微镜
    自备试剂 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),二甲苯,乙醇,DNase I,PBS(E-IR-R187),ddH2O,苏木素(E-IR-R120),中性树胶(E-IR-R118)
    保存温度 -20°C, shading light. Refer to the Manual.
    运输条件 冰袋
    保质期 12 months

    操作指南

    客户发表文献

    1. 文献: Effects of hydrogen-rich saline in neuroinflammation and mitochondrial dysfunction in rat model of sepsis-associated encephalopathy
    2. 文献: Regulation Networks of Non-Coding RNA-Associated ceRNAs in Cisplatin-Induced Acute Kidney Injury
    3. 文献: Scutellarin Protects Against Mitochondrial Reactive Oxygen Species-Dependent NLRP3 Inflammasome Activation to Attenuate Intervertebral Disc Degeneration.
    4. 文献: Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury
      样品类型: Serum,Plasma,Urine
    5. 文献: Oxidized high-density lipoprotein enhances endocrine disorders and ovarian damage in rats
    6. 文献: Influence of hyperocclusion on the remodeling of gingival tissues
    7. 文献: Rifaximin protects SH-SY5Y neuronal cells from iron overload-induced cytotoxicity via inhibiting STAT3/NF-κB signaling
    8. 文献: The protective effect of zinc on morphine-induced testicular toxicity via p53 and Akt pathways: An in vitro and in vivo approach
      样品类型: Cell culture supernatant
    9. 文献: May Argyrophilic Nucleolar Organizer Regions Be Used as a Biomarker for the Detection of the Degree of Ischemic Damage Instead of Tunel in Testicular Torsion?
    10. 文献: Prednisolone induces osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway
    11. 文献: In Vitro and In Vivo Pretreatment with Selenium Mitigates Tetrahydrocannabinol-Induced Testicular Cell Apoptosis: the Role of AKT and p53 Pathways
      样品类型: Cell sample
    12. 文献: Silencing circRNA LRP6 down‐regulates PRMT1 to improve the streptozocin‐induced pancreatic β-cell injury and insulin secretion by sponging miR-9-5p
      样品类型: Cell sample
    13. 文献: The effects of the renin inhibitor aliskiren on neuronal toxic damage
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