TUNEL In Situ Apoptosis Kit (HRP-DAB Method)
TUNEL原位细胞凋亡检测试剂盒(HRP-DAB显色法)
规格: | / 20Assays / 50Assays / 100Assays |
价格: | / ¥1650 / ¥2850 / ¥4620 |
货号:E-CK-A331
检测原理
细胞在发生凋亡时,会激活一些DNA内切酶,剪断核小体间的基因组DNA,暴露的3’-OH可在末端脱氧核酸转移酶(TdT)的催化下加上生物素标记的dUTP,辣根过氧化物酶(HRP)标记的Streptavidin (Streptavidin-HRP)可与之结合,在HRP的催化下通过DAB显色可观测到凋亡细胞,从而可通过普通光学显微镜检测到细胞的凋亡。由于正常的或正在增殖的细胞中几乎没有DNA的断裂,因而没有3'-OH形成,很少能够被标记。这就是TUNEL(TdT-mediated dUTP Nick-End Labeling)法检测细胞凋亡的原理。
基本信息
应用类型 | 细胞凋亡-DNA片段化 |
检测方法 | DAB显色 |
样本类型 | 石蜡切片,冰冻切片,细胞爬片,细胞涂片 |
检测时间 | 3 hours |
检测仪器 | 普通光学显微镜 |
自备试剂 | 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),二甲苯,乙醇,DNase I,PBS(E-IR-R187),ddH2O,苏木素(E-IR-R120),中性树胶(E-IR-R118) |
保存温度 | -20°C, shading light. Refer to the Manual. |
运输条件 | 冰袋 |
保质期 | 12 months |
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