Western Blot Detection Kit
Western Blot试剂盒
规格: | / 50 Assays |
价格: | / ¥1020 |
货号:E-IR-R304A
应用: WB
储存: 室温/ 2~8°C/-20°C,详见说明书
Western Blot试剂盒( E-IR-R304)
WB,Western Blot(免疫印迹实验)是生物科学领域检测蛋白质表达最常用和最经典的实验方法。然而由于不同实验人员所用试剂来源、操作方法和经验的区别,导致大量WB实验结果不符合文章发表要求。Elabscience专注于免疫学领域多年,积累了大量的WB操作经验。为帮助科研人员解决上述问题,现特推出Western Blot检测试剂盒。试剂盒内为经典WB实验所需试剂,包含了从蛋白提取到ECL底物曝光在内的所有试剂,具有操作简便、检测灵敏度高、背景低、系统稳定性强等优点,并提供详尽的操作说明和专业的技术支持,为您顺利做出理想的Western Blot实验结果提供了便捷和保障。
由于PVDF膜有两种孔径,分别适用于转不同分子量大小的目的蛋白;为了满足不同实验需求,我们设计了两种规格的Western Blot试剂盒:
E-IR-R304A(PVDF膜0.45 μm孔径)建议用于检测分子量大于20 kDa的蛋白质;
E-IR-R304B(PVDF膜0.22 μm孔径)建议用于检测分子量小于20 kDa的蛋白质。
- E-IR-R304A
E-IR-R304A(PVDF膜0.45 μm孔径)建议用于检测分子量大于20 kDa的蛋白质
提供产品:
产品 | 货号 | 规格 | 储存温度 | 保质期 |
---|---|---|---|---|
RIPA裂解液(含PMSF和原钒酸钠) | E-BC-R327 | 5mL/50μL+50μL | -20℃ | 12个月 |
BCA蛋白浓度测定试剂盒 | E-BC-K318 | 48T | RT | 12个月 |
ECL发光检测液 | E-IR-R307 | 15mL/15mL | 4℃ | 12个月 |
预染蛋白Marker(10-180kDa) | E-BC-R273 | 25µL | -20℃ | 12个月 |
Goat Anti-Rabbit IgG (H+L) HRP | E-AB-1003 | 20µL | -20℃ | 12个月 |
脱脂奶粉 | E-BC-R337 | 15g | 室温 | 12个月 |
PBS缓冲液(10×) | E-BC-R187 | 100mL | 室温 | 12个月 |
5 X SDS上样缓冲液 | E-BC-R288 | 1mL×2 | -20℃ | 12个月 |
电泳缓冲液(10×) | E-BC-R331 | 125mL×2 | 室温 | 12个月 |
转膜缓冲液(10×) | E-BC-R333 | 125mL×2 | 4℃ | 12个月 |
TBST缓冲液(10×) | E-BC-R335 | 125mL×2 | 4℃ | 12个月 |
PVDF膜(0.45μm, 8.5 × 6cm) | E-BC-R266 | 5片 | 室温 | 12个月 |
赠送试剂:
产品 | 货号 | 规格 | 储存温度 | 保质期 |
---|---|---|---|---|
Goat Anti-Mouse IgG(H+L) HRP | E-AB-1001 | 20µL | -20℃ | 12个月 |
需自备试剂:甲醇
使用说明:
本试剂盒提供的产品可供完成5块凝胶(约45个样本)对应的 Western Blot 试验。
收到该试剂盒后,请按照说明书所提供的保存条件进行保存。
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