Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
谷丙转氨酶(ALT/GPT)比色法测试盒
规格: | / 48T(18 samples) / 96T(42 samples) |
价格: | / ¥200 / ¥320 |
货号:E-BC-K235-M
检测仪器及波长: 酶标仪(500-520 nm)
样本类型: 血清(浆);动物组织;细胞;细胞上清
检测原理
在37℃、pH7.4条件下,谷丙转氨酶(ALT)催化丙氨酸与α-酮戊二酸之间的氨基转换反应,生成丙酮酸和谷氨酸,反应时间过后,加入苯肼,与丙酮酸生成苯腙,苯腙在碱性条件下呈红棕色。
关键信息
指标简称 | ALT,GPT |
样本类型 | 血清(浆);动物组织;细胞;细胞上清 |
灵敏度 | 0.75 IU/L |
检测范围 | 0.75-72.3 IU/L |
精密度 | 批间差:9%批内差:5.3% |
检测仪器及波长 | 酶标仪 |
保存条件 | 2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择差异较大的2-3个样本稀释成不同浓度进行预实验,结合本试剂盒的检测范围(0.75-72.30 IU/L),选取最佳稀释倍数,进行正式批量实验。不同样本的稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
不稀释 |
10%大鼠脑匀浆 |
不稀释 |
人血浆 |
不稀释 |
10%大鼠心匀浆 |
不稀释 |
猪血清 |
不稀释 |
10%大鼠肝匀浆 |
40-60 |
大鼠血清 |
不稀释 |
10%大鼠肾匀浆 |
不稀释 |
注:稀释液为生理盐水(0.9% NaCl)或PBS(0.01 M,pH 7.4)。
相关产品
货号 | 产品名称 |
---|---|
E-BC-K236-M | 谷草转氨酶(AST/GOT)比色法测试盒 |
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