Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)
白蛋白(ALB)比色法测试盒(溴甲酚绿法)
规格: | / 48T(32 samples) / 96T(80 samples) |
价格: | / ¥200 / ¥320 |
货号:E-BC-K057-M
检测仪器及波长: 酶标仪(620-640 nm)
样本类型: 动物血清(浆)
检测原理
溴甲酚绿(BCG)是一种应用较广泛的蛋白染色剂,在pH值为4.0-4.2时,白蛋白与溴甲酚绿结合,溶液由黄色变绿色,且颜色与白蛋白浓度正呈比例关系,通过比色可计算出血清中白蛋白的含量。
关键信息
指标简称 | ALB |
样本类型 | 动物血清(浆) |
灵敏度 | 0.08 g/L |
检测范围 | 0.08-15 g/L |
精密度 | 批间差:4.6%批内差:1.5% |
检测仪器及波长 | 酶标仪 |
保存条件 | Reagent 1 2-8℃,Reagent 2 -20℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3个预期差异大的样本稀释成不同浓度进行预实验,结合本试剂盒的线性范围(0.08-15 g/L),选取最佳稀释倍数,进行正式批量实验。不同样本的稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
8-15 |
小鼠血浆 |
8-15 |
人血浆 |
8-15 |
大鼠血清 |
8-15 |
HepG2细胞上清 |
不稀释 |
|
|
注:稀释液为生理盐水(0.9% NaCl)或PBS(0.01 M,pH 7.4)。
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