Alkaline Phosphatase (ALP) Activity Assay Kit
碱性磷酸酶(ALP)比色法测试盒
| 规格： | / 50 assays(48 samples) / 100 assays(96 samples) |
| 价格： | / ￥320 / ￥580 |
货号：E-BC-K091-S
检测仪器及波长: 紫外-可见光分光光度计（520 nm）
样本类型: 动物血清(浆);体液;组织及细胞
检测原理
在pH=10的反应液中，碱性磷酸酶催化磷酸苯二钠水解，生成游离酚和磷酸。酚在碱性溶液中与4-氨基安替吡啉结合，并经铁氰化钾氧化生成红色的醌的衍生物，根据红色的深浅计算酶活的高低。
关键信息
| 指标简称 | ALP |
| 样本类型 | 动物血清(浆);体液;组织及细胞 |
| 灵敏度 | 0.2 金式单位/100 mL |
| 检测范围 | 0.2-55.6金式单位/100 mL |
| 精密度 | 批间差：5.6%批内差：2.1% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 3个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的检测范围（0.2-55.6金式单位/100 mL），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
10%小鼠肾匀浆 |
30-50 |
|
人尿液 |
不稀释 |
10%小鼠肝匀浆 |
不稀释 |
|
大鼠血清 |
不稀释 |
HepG2细胞 |
不稀释 |
|
细胞上清 |
不稀释 |
10%小鼠脑匀浆 |
不稀释 |
注：稀释液为PBS（0.01 M，pH 7.4）或生理盐水（0.9% NaCl）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-K094-S | 酸性磷酸酶(ACP)比色法测试盒 |
客户发表文献
- 文献: A prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells
样品类型: serum - 文献: Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis
样品类型: plasma - 文献: The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis
- 文献: BMSC exosome-enriched acellular fish scale scaffolds promote bone regeneration
样品类型: BMSCs - 文献: IGF2BP2, an RNA-binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA
- 文献: Indole-3-Carboxaldehyde Restores Gut Mucosal Integrity and Protects from Liver Fibrosis in Murine Sclerosing Cholangitis
样品类型: serum - 文献: Carthami flos extract against carbon tetrachloride-induced liver fibrosis via alleviating angiogenesis in mice
样品类型: serum - 文献: Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism
样品类型: serum - 文献: Phillygenin Attenuates Carbon Tetrachloride-Induced Liver Fibrosis via Modulating Inflammation and Gut Microbiota
样品类型: serum - 文献: Degradation and biological performance of porous osteomimetic biphasic calcium phosphate in vitro and in vivo
样品类型: MG63 cell - 文献: Coupling Induction of Osteogenesis and Type H Vessels by Pulsed Electromagnetic Fields in Ovariectomy-induced Osteoporosis in Mice
样品类型: serum - 文献: Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis
样品类型: serum - 文献: Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence
样品类型: serum - 文献: Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis
样品类型: MC3T3-E1 cell - 文献: Cellular responses of histatin-derived peptides immobilized titanium surface using a tresyl chloride-activated method
样品类型: Cell lysate - 文献: miR-215-5p regulates osteoporosis development and osteogenic differentiation by targeting XIAP
样品类型: hBMSC - 文献: Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion :
样品类型: serum - 文献: San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress
样品类型: hepatic tissue,serum - 文献: Mechanisms by which electroacupuncture‑mediated histone acetylation mitigates bone loss in rats with ovariectomy‑induced osteoporosis
样品类型: Serum - 文献: Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress
样品类型: liver - 文献: Supplementation of non-fermented and fermented goji berry (Lycium barbarum) improves hepatic function and corresponding lipid metabolism via their anti-inflammatory and antioxidant properties in high fat-fed rats
样品类型: serum - 文献: Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis
样品类型: Serum - 文献: Noninvasive Visualization of Obesity-Boosted Inflammation in Orthotopic Pancreatic Ductal Adenocarcinoma Using an Octapod Iron Oxide Nanoparticle
样品类型: Serum - 文献: Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats
样品类型: plasma - 文献: Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects
样品类型: plasma
