ATP Colorimetric Assay Kit
ATP含量比色法测试盒
规格: | / 48T(22 samples) / 96T(46 samples) |
价格: | / ¥450 / ¥800 |
货号:E-BC-K157-M
检测仪器及波长: 酶标仪(630-640 nm,最适检测波长636 nm)
样本类型: 动物组织
检测原理
肌酸激酶催化肌酸和 ATP 反应生成磷酸肌酸,通过比色法检测磷酸肌酸的含量,以此反应出ATP的含量。
关键信息
指标简称 | ATP |
样本类型 | 动物组织 |
灵敏度 | 0.01 mmol/L |
检测范围 | 0.03-1.5 mmol/L |
精密度 | 批间差:5.4%批内差:5.1% |
检测仪器及波长 | 酶标仪 |
特殊仪器 | 水浴锅(100℃) |
保存条件 | Reagent 4 -20℃,其余2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择差异较大的2-3例样本稀释成不同浓度进行预实验,结合检测范围:0.03-1.5 mmol/L,不同样本的稀释如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
10%大鼠肌肉组织 |
2-4 |
10%大鼠肾脏组织 |
2-4 |
10%大鼠肝脏组织 |
2-4 |
10%大鼠肺组织 |
2-4 |
10%大鼠脑组织 |
2-4 |
|
|
注:稀释液为去离子水
相关产品
货号 | 产品名称 |
---|---|
E-BC-F002 | ATP含量化学发光法测试盒 |
E-BC-K157-S | ATP含量比色法测试盒 |
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