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ATP Colorimetric Assay Kit

ATP含量比色法测试盒

规格: / 48T(22 samples) / 96T(46 samples)
价格: / ¥450 / ¥800

货号:E-BC-K157-M

检测仪器及波长: 酶标仪(630-640 nm,最适检测波长636 nm)

样本类型: 动物组织

  • 详情
  • 文献(13)
  • 检测原理

    肌酸激酶催化肌酸和 ATP 反应生成磷酸肌酸,通过比色法检测磷酸肌酸的含量,以此反应出ATP的含量。

    关键信息

    指标简称 ATP
    样本类型 动物组织
    灵敏度 0.01 mmol/L
    检测范围 0.03-1.5 mmol/L
    精密度 批间差:5.4%批内差:5.1%
    检测仪器及波长 酶标仪
    特殊仪器 水浴锅(100℃)
    保存条件 Reagent 4 -20℃,其余2-8℃
    有效期 6个月

    样本稀释倍数

           在正式检测前,需选择差异较大的2-3例样本稀释成不同浓度进行预实验,结合检测范围:0.03-1.5 mmol/L,不同样本的稀释如下表(仅供参考):

    样本

    稀释倍数

    样本

    稀释倍数

    10%大鼠肌肉组织

    2-4

    10%大鼠肾脏组织

    2-4

    10%大鼠肝脏组织

    2-4

    10%大鼠肺组织

    2-4

    10%大鼠脑组织

    2-4

    :稀释液为去离子水

    客户发表文献

    1. 文献: Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer
    2. 文献: Autophagy in Rat Müller Glial Cells Is Modulated by the Sirtuin 4/AMPK/mTOR Pathway and Induces Apoptosis under Oxidative Stress
      样品类型: rMC-1 cell
    3. 文献: Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model
      样品类型: mammary adenocarcinoma tissue
    4. 文献: Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation
      样品类型: nucleus pulposus(NP) cell
    5. 文献: N-acetyl-L-cysteine ameliorates mitochondrial dysfunction in ischemia/reperfusion injury via attenuating Drp-1 mediated mitochondrial autophagy
      样品类型: brain tissue
    6. 文献: Antibacterial activity and action mechanism of flavonoids against phytopathogenic bacteria
    7. 文献: Metabolic Phenotype Intricacies on Altered Glucose Metabolism of Breast Cancer Cells upon Glut-1 Inhibition and Mimic Hypoxia In Vitro
      样品类型: SKBR-3 breast cancer cell
    8. 文献: Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells
      样品类型: HCT116 cell
    9. 文献: Salvianolic acid A promotes mitochondrial biogenesis and function via regulating the AMPK/PGC‑1α signaling pathway in HUVECs
      样品类型: Primary HUVECs
    10. 文献: San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress
      样品类型: hepatic tissue
    11. 文献: Honokiol Reduces Mitochondrial Dysfunction and Inhibits Apoptosis of Nerve Cells in Rats with Traumatic Brain Injury by Activating the Mitochondrial Unfolded Protein Response
      样品类型: brain tissues
    12. 文献: CircSEC31A Promotes the Malignant Progression of Non-Small Cell Lung Cancer Through Regulating SEC31A Expression via Sponging miR-376a
      样品类型: Cell sample
    13. 文献: Simple neutralization test report: Do probiotics contribute to COVID-19 therapy?
      样品类型: PBMCs
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