Catalase (CAT) Activity Assay Kit
过氧化氢酶(CAT) 比色法测试盒
| 规格： | / 50 assays(25 samples) / 100 assays(50 samples) |
| 价格： | / ￥380 / ￥680 |
货号：E-BC-K031-S
检测仪器及波长: 紫外-可见光分光光度计（405 nm）
样本类型: 血清;血浆;培养细胞及动植物组织样本
检测原理
过氧化氢酶催化H2O2分解为氧和水，钼酸铵能够迅速中止CAT分解H2O2的反应，而剩余的H2O2与钼酸铵作用产生一种淡黄色的络合物，在波长405 nm处测定其生成量，可计算出CAT的活力。
关键信息
| 指标简称 | CAT |
| 样本类型 | 血清;血浆;培养细胞及动植物组织样本 |
| 灵敏度 | 0.27 U/mL |
| 检测范围 | 0.27-155.4 U/mL |
| 精密度 | 批间差：5.1%批内差：3.1% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
本试剂盒的检测范围在0.27-155.4 U/mL，测试前选择2-3个预期差异大的样本进行预实验以确定最佳取样浓度。不同样本稀释比例范围如下表（仅供参考）：
|
样本 |
稀释倍数 |
上样量 |
|
10%大鼠肝匀浆 |
25-50 |
50 μL |
|
10%大鼠肾匀浆 |
10-25 |
50 μL |
|
10%大鼠脑匀浆 |
5-10 |
50 μL |
|
人血清 |
不稀释 |
100 μL |
|
293T细胞上清 |
不稀释 |
100 μL |
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-K025-S | 丙二醛(MDA)比色法测试盒(TBA法) |
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