Flag-Tag Polyclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-40525
宿主: Rabbit
反应性: All
应用: WB, IF,IP
Overview
| Synonyms | DDDDK epitope tag,DDDDK epitope tag |
| Source | Rabbit |
| Reactivity | All |
| Immunogen | Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin. |
| Application | WB(Detection kit: E-IR-R304), IF,IP |
| Recommended dilution | WB,,1:2000-5000;IP,,3ug/sample;IF,,1:6000-12000; |
| Concentration | 4mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
| Background | FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This |
Images
Western blotting with Anti-FLAG rabbit polyclonal antibody at dilution of 1:1000.Lane1: FLAG tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate
Immunofluorescent analysis of Hela cells transfected with the plasmid overexpressing Flag fusion Protein, using anti-Flag -Tag polyclonal antibody at 1:200 dilution.
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Polyclonal Antibody at dilution of 1:12000.
IP Result of 293F cells transfected with FLAG-Tag fusion protein,using anti-FLAG-Tag rabbit antibody . Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-FLAG-Tag rabbit antibody
