H2S Colorimetric Assay Kit
硫化氢(H2S)比色法测试盒
| 规格： | / 48T(31 samples) / 96T(79 samples) |
| 价格： | / ￥420 / ￥720 |
货号：E-BC-K355-M
检测仪器及波长: 酶标仪(660-670 nm，最佳检测波长为665 nm)
样本类型: 血清;血浆;动物组织
检测原理
H2S可被乙酸盐溶液吸收，形成ZnS沉淀；在碱性溶液中溶解，在Fe3+存在情况下，可形成稳定的亚甲基蓝，亚甲基蓝在665nm处有最大吸收峰，通过测定其吸光值可计算H2S含量。
关键信息
| 指标简称 | H2S |
| 样本类型 | 血清;血浆;动物组织 |
| 灵敏度 | 2.75 μmol/L |
| 检测范围 | 4.86-100 μmol/L |
| 精密度 | 批间差：9.9%批内差：3.1% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择预期差异较大的2-3个样本稀释成不同浓度进行预实验，结合检测范围：6.82-100 μmol/L，不同样本的稀释如下表(仅供参考)：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血浆 |
不稀释 |
10%大鼠肾脏组织匀浆 |
不稀释 |
|
小鼠血浆 |
不稀释 |
10%大鼠脑组织匀浆 |
不稀释 |
|
10%大鼠脾脏组织匀浆 |
不稀释 |
|
|
注：稀释液为生理盐水(0.9% NaCl)。
客户发表文献
- 文献: A hydrogen sulphide-responsive and depleting nanoplatform for cancer photodynamic therapy
样品类型: liver - 文献: A dual lock-and-key two photon fluorescence probe in response to hydrogen peroxide and viscosity: Application in cellular imaging and inflammation therapy
样品类型: H9C2 cells - 文献: Hyperglycemia and Loss of Redox Homeostasis in COVID-19 Patients
样品类型: plasma - 文献: The role of l-cysteine/Hydrogen sulfide pathway on β3-Adrenoceptor- induced relaxation in mouse gastric fundus
样品类型: gastric fundal tissue - 文献: Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism
样品类型: Plasma,Tissue homogenate - 文献: Co-administration of sodium hydrosulfide and tadalafil modulates hypoxia and oxidative stress on bladder dysfunction in a rat model of bladder outlet obstruction.
样品类型: bladder tissue - 文献: Involvement of RhoA/Rho-kinase in l-cysteine/H2S pathway-induced inhibition of agonist-mediated corpus cavernosal smooth muscle contraction
样品类型: Tissue homogenate - 文献: Evaluation of combined therapeutic effects of hydrogen sulfide donor sodium hydrogen sulfide and phosphodiesterase type‐5 inhibitor tadalafil on erectile dysfunction in a partially bladder outlet obstructed rat model
样品类型: Tissue homogenate - 文献: Effects of hydrogen sulphide donor, sodium hydrosulphide treatment on the erectile dysfunction in L‐NAME‐induced hypertensive rats
样品类型: Tissue homogenate
