L-Lactic Acid (LA) Colorimetric Assay Kit
L-乳酸(LA)比色法测试盒
规格: | / 48T(32 samples) / 96T(80 samples) |
价格: | / ¥620 / ¥1100 |
货号:E-BC-K044-M
检测仪器及波长: 酶标仪(520-540 nm,最适检测波长530 nm)
样本类型: 血清(浆);细胞上清;组织;细胞
检测原理
以氧化型辅酶Ⅰ(NAD+)为氢受体,乳酸脱氢酶(LDH)催化乳酸脱氢产生丙酮酸,使NAD+转化为还原型辅酶Ⅰ(NADH)。其中N-甲基吩嗪甲基硫酸盐(PMS)递氢使氯化硝基四氮唑蓝(NBT)还原为紫色呈色物,呈色物的吸光度在530 nm时与乳酸含量呈线性关系。
关键信息
样本类型 | 血清(浆);细胞上清;组织;细胞 |
灵敏度 | 0.10 mmol/L |
检测范围 | 0.12-7.0 mmol/L |
精密度 | 批间差:3.5%批内差:1.4% |
检测仪器及波长 | 酶标仪 |
保存条件 | 2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择样本之间差异较大的2-3例稀释成不同浓度进行预实验,不同样本稀释比例如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
2-5 |
HepG2细胞上清 |
不稀释 |
10%大鼠肾脏组织 |
1-3 |
HepG2细胞 |
不稀释 |
10%大鼠脑组织 |
不稀释 |
|
|
注:稀释液为生理盐水(0.9% NaCl)或PBS(0.01 M,pH 7.4)
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