MDA(Malondialdehyde) ELISA Kit
丙二醛(MDA)酶联免疫吸附测定试剂盒
规格: | / 96T / 48T / 96T*5 |
价格: | / ¥2400 / ¥1650 / ¥10200 |
货号:E-EL-0060
反应性: 通用
检测范围: 31.25~2000 ng/mL
灵敏度: 18.75 ng/mL
用途
该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中MDA浓度。
检测原理
本试剂盒采用竞争ELISA法。用MDA抗原包被于酶标板上,实验时样品或标准品中的MDA与包被的MDA竞争生物素标记的抗MDA单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,MDA浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中MDA的浓度。
反应类型 | 竞争法 |
规格 | 96T |
反应时间 | 2.0h |
反应性 | 通用 |
检测方法 | Colormetric |
检测范围 | 31.25-2000 ng/mL |
灵敏度 | 18.75 ng/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆或其他相关生物液体 |
特异性
可检测样本中的MDA,且与其它相关蛋白无明显交叉反应。
重复性
板内,板间变异系数均<10%。
典型数据
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
(ng/mL) | O.D | Average |
---|---|---|
2000 | 0.352 0.398 |
0.375 |
1000 | 0.498 0.508 |
0.503 |
500 | 0.741 0.709 |
0.725 |
250 | 1.058 1.068 |
1.063 |
125 | 1.507 1.481 |
1.494 |
62.5 | 1.942 1.92 |
1.931 |
31.25 | 2.284 2.284 |
2.284 |
0 | 2.804 2.818 |
2.811 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) |
109.60 | 308.70 | 848.80 | 111.50 | 321.00 | 892.50 |
Standard
deviation |
6.10 | 17.30 | 37.30 | 7.00 | 17.00 | 46.40 |
C V (%) | 5.57 | 5.60 | 4.39 | 6.28 | 5.30 | 5.20 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum (n=5) | 92-103 | 97 |
EDTA plasma (n=5) | 94-107 | 99 |
Cell culture media (n=5) | 93-110 | 100 |
线性
分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 84-99 | 89-101 | |
Average (%) | 99 | 91 | 95 | |
1:4 | Range (%) | 82-95 | 88-103 | 100-113 |
Average (%) | 89 | 95 | 105 | |
1:8 | Range (%) | 86-97 | 87-102 | 99-111 |
Average (%) | 92 | 94 | 105 | |
1:16 | Range (%) | 85-96 | 87-99 | 94-110 |
Average (%) | 91 | 92 | 100 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。
中文名称 | 英文名称 | 规格 | 保存条件 |
---|---|---|---|
ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃,可存放6个月 |
冻干标准品 | Reference Standard | 2支 | |
浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光),可存放6个月 |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
封板覆膜 | Plate Sealer | 5 张 | |
产品说明书 | Product Description | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 |
说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。
试验所需自备物品
- 1.酶标仪(450nm波长滤光片)
- 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3.37℃恒温箱,双蒸水或去离子水
- 4.吸水纸
操作步骤
|
1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37℃孵育90分钟 |
2. 弃掉板内液体,洗板3次 | |
3. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次 | |
4. 每孔加入90μL底物溶液,37℃孵育15分钟 | |
5. 每孔加入50μL终止液 | |
6. 立即在450nm波长下读数,处理数据 |
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样品类型: Serum - 文献: The effects of six months Persicaria minor extract supplement among older adults with mild cognitive impairment: a double-blinded, randomized, and placebo-controlled trial
样品类型: Serum - 文献: Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies
样品类型: Serum - 文献: Possible combined effect of perindopril and Azilsartan in an experimental model of dementia in rats
样品类型: Tissue homogenate - 文献: Effect of enriched environment and predictable chronic stress on spatial memory in adolescent rats: Predominant expression of BDNF, nNOS, and interestingly malondialdehyde in the right hippocampus
样品类型: hippocampus - 文献: Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis
样品类型: serum - 文献: Puerarin reduces ischemia/reperfusion-induced myocardial injury in diabetic rats via upregulation of vascular endothelial growth factor A/angiotensin-1 and suppression of apoptosis
样品类型: plasma - 文献: Quercetin prevents chronic kidney disease on mesangial cells model by regulating inflammation, oxidative stress, and TGF-β1/SMADs pathway
样品类型: SV40 MES 13 cell - 文献: Carvacrol treatment opens Kir6.2 ATP-dependent potassium channels and prevents apoptosis on rat testis following ischemia–reperfusion injury model
样品类型: serum - 文献: Administration of Donor-Derived Nonexpanded Adipose Stromal Vascular Fraction Attenuates Ischemia-Reperfusion Injury in Donation After Cardiac Death Rat Renal Transplantation
样品类型: serum - 文献: Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats
样品类型: Serum,Tissue homogenate - 文献: Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy
样品类型: Heart tissue - 文献: Protective effects of curcumin and beta-carotene on cisplatin-induced cardiotoxicity: An experimental rat model
样品类型: Tissue homogenate - 文献: Myricetin protects against diet-induced obesity and ameliorates oxidative stress in C57BL/6 mice
样品类型: Serum - 文献: Ozone Partially Decreases Axonal and Myelin Damage in an Experimental Sciatic Nerve Injury Model
样品类型: Serum - 文献: Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti‑inflammatory effects, and inhibition of the p38‑MAPK mechanism
样品类型: Serum - 文献: Nobiletin ameliorates isoflurane-induced cognitive impairment via antioxidant, anti-inflammatory and anti-apoptotic effects in aging rats
样品类型: Serum - 文献: Toxic effects of subchronic oral acetamiprid exposure in rats:
样品类型: plasma,renal,liver - 文献: Citrus hystrix leaf extract attenuated diabetic‐cataract in STZ‐rats
样品类型: serum - 文献: AMPK activation restores ischemic post‑conditioning cardioprotection in STZ‑induced type 1 diabetic rats: Role of autophagy
样品类型: Serum - 文献: Protective effect by low-intensity downhill running training against muscle damage and oxidative stress after high-intensity downhill running in rats
样品类型: Plasma,vastus intermedius muscle tissue - 文献: Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats
样品类型: Tissue homogenate - 文献: Astaxanthin protects against hearing impairment in diabetic rats
样品类型: serum,cochlea - 文献: Effects of Achillea millefolium on cisplatin induced ocular toxicity: an experimental study
样品类型: ocular - 文献: Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function
样品类型: Tissue homogenate - 文献: Lipid storage droplet protein 5 reduces sodium palmitate‑induced lipotoxicity in human normal liver cells by regulating lipid metabolism‑related factors
样品类型: LO2 cells - 文献: Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice
样品类型: Plasma,Tissue homogenate - 文献: Pyrroloquinoline Quinone Inhibits Oxidative Stress in Rats with Diabetic Nephropathy
样品类型: kidney tissue,NRK-52E cell - 文献: Embelin inhibits abdominal aortic aneurysm through decreasing IL‑6‑induced STAT3 and NF‑κB inactivation
样品类型: Aortic tissues - 文献: The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer
样品类型: Plasma - 文献: The Induction of Antioxidant Catalase Enzyme With Decrease of Plasma Malonidialdehyde: An Important Reactive Oxidative Species Inhibiting Mechanism
样品类型: Plasma - 文献: The role of polysaccharide peptide of Ganoderma lucidum as a potent antioxidant against atherosclerosis in high risk and stable angina patients
样品类型: plasma - 文献: Protective effects of Labisia pumila against neuropathy in a diabetic rat model
样品类型: serum - 文献: Correlation between C-reactive Protein with Malondialdehyde in Systemic Lupus Erythematosus Patients
样品类型: Serum - 文献: Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain
样品类型: serum,sciatic nerves - 文献: Vitamin E protected the mouse adrenal gland against immobilization stress.
样品类型: adrenal tissue