MS Mouse IL-12(Interleukin 12) ELISA Kit
MS-小鼠白介素12(IL-12)酶联免疫吸附测定试剂盒
规格: | / 96T / 48T / 96T*5 |
价格: | / ¥3080 / ¥2180 / ¥13860 |
货号:E-MSEL-M0004
反应性: 小鼠
检测范围: 0.31~20 pg/mL
灵敏度: 0.19 pg/mL
MS系列
MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。
用途
该试剂盒用于体外定量检测小鼠serum, plasma and other biological fluids中IL-12浓度
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-12抗体包被于酶标板上,实验时样品或标准品中的小鼠IL-12会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠IL-12抗体和辣根过氧化物酶标记的亲和素。抗小鼠IL-12抗体与结合在包被抗体上的小鼠IL-12结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-12浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-12的浓度。
反应类型 | 夹心法 |
规格 | 96T |
反应时间 | 3.5h |
反应性 | 小鼠 |
检测方法 | Colormetric |
检测范围 | 0.31—20 pg/mL |
灵敏度 | 0.19 pg/mL |
样本体积 | 25μL |
样本类型 | serum, plasma and other biological fluids |
特异性
可检测样本中的小鼠IL-12,且与其它相关蛋白无明显交叉反应。
重复性
板内,板间变异系数均<10%。
典型数据
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
(pg/mL) | O.D | Average | Corrected |
---|---|---|---|
20 | 2.444 2.362 |
2.403 | 2.335 |
10 | 2.017 2.009 |
2.013 | 1.945 |
5 | 1.324 1.414 |
1.369 | 1.301 |
2.5 | 0.832 0.834 |
0.833 | 0.765 |
1.25 | 0.467 0.477 |
0.472 | 0.404 |
0.63 | 0.275 0.278 |
0.276 | 0.209 |
0.31 | 0.183 0.183 |
0.183 | 0.115 |
0 | 0.068 0.067 |
0.067 | -- |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) |
0.94 | 2.22 | 8.84 | 0.83 | 2.17 | 8.86 |
Standard
deviation |
0.05 | 0.11 | 0.42 | 0.05 | 0.11 | 0.42 |
C V (%) | 5.72 | 4.95 | 4.74 | 5.80 | 4.96 | 4.74 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum (n=5) | 87-97 | 93 |
EDTA plasma (n=5) | 86-98 | 90 |
Cell culture media (n=5) | 93-105 | 99 |
线性
分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 88-103 | 98-108 | 94-104 |
Average (%) | 96 | 103 | 98 | |
1:4 | Range (%) | 99-107 | 91-102 | 88-100 |
Average (%) | 102 | 97 | 92 | |
1:8 | Range (%) | 95-109 | 94-102 | 96-106 |
Average (%) | 103 | 98 | 100 | |
1:16 | Range (%) | 98-105 | 84-94 | 99-106 |
Average (%) | 101 | 89 | 101 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。
中文名称 | 英文名称 | 规格 | 保存条件 |
---|---|---|---|
ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃,可存放6个月 |
冻干标准品 | Reference Standard | 2 支 | |
浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光),可存放6个月 |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
封板覆膜 | Plate Sealer | 5 张 | |
产品说明书 | Product Description | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 |
说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。
试验所需自备物品
- 1. 酶标仪(450nm波长滤光片)
- 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3. 37℃恒温箱,双蒸水或去离子水
- 4. 吸水纸
操作步骤
|
1.对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟 |
2.弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟 | |
3.弃掉板内液体,洗板3次 | |
4.每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次 | |
5.每孔加入90μL底物溶液,37℃孵育15分钟 | |
6.每孔加入50μL终止液 | |
7.立即在450nm波长下读数,处理数据 |
客户发表文献
- 文献: Tumor Cell Nanovaccines based on Genetically Engineered Antibody-anchored Membrane
样品类型: bone-marrow-derived dendritic cells (BMDCs) - 文献: Quantitative Photoacoustic Diagnosis and Precise Treatment of Inflammation In Vivo Using Activatable Theranostic Nanoprobe
样品类型: Serum - 文献: Novel combination strategy of high intensity focused ultrasound (HIFU) and checkpoint blockade boosted by bioinspired and oxygen-supplied nanoprobe for multimodal imaging-guided cancer therapy
样品类型: Serum - 文献: Biomimetic nanoprobe-augmented triple therapy with photothermal, sonodynamic and checkpoint blockade inhibits tumor growth and metastasis
样品类型: tumor cell - 文献: Expression of miRNA-29 in Pancreatic β Cells Promotes Inflammation and Diabetes via TRAF3
样品类型: Cell sample - 文献: RNF138 inhibits late inflammatory gene transcription through degradation of SMARCC1 of the SWI/SNF complex.
样品类型: serum,RAW264.7 cell - 文献: Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function
样品类型: colonic tissue - 文献: Recombinant Listeria ivanovii strain expressing listeriolysin O in place of ivanolysin O might be a potential antigen carrier for vaccine construction
样品类型: splenocytes - 文献: Chitosan-Poly(Acrylic Acid) Nanoparticles Loaded with R848 and MnCl2 Inhibit Melanoma via Regulating Macrophage Polarization and Dendritic Cell Maturation
样品类型: serum,CD8+ T cell,BMDCs - 文献: Norcantharidin liposome emulsion hybrid delivery system enhances PD-1/PD-L1 immunotherapy by agonizing the non-canonical NF-κB pathway
- 文献: Resolving the hepatic fibrosis via suppressing oxidative stress and inflammatory response by novel hyaluronic acid modified nanocomplex
样品类型: serum - 文献: Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis
样品类型: serum,lymphocytes - 文献: λ-Carrageenan improves the antitumor effect of dendritic cellbased vaccine
样品类型: Dendritic cell - 文献: Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
样品类型: 4T1 cell - 文献: Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
样品类型: Splenocyte - 文献: Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice
样品类型: serum,Splenocyte - 文献: Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis
样品类型: colon tissue,serum - 文献: A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC
样品类型: Cell culture supernatant - 文献: Toxoplasma gondii ROP16I Deletion: The Exacerbated Impact on Adverse Pregnant Outcomes in Mice
样品类型: placental tissue,serum,spleen cell - 文献: Glycyrrhiza uralensis water extract enhances dendritic cell maturation and antitumor efficacy of HPV dendritic cell-based vaccine
样品类型: Cell culture supernatant - 文献: Defibrotide inhibits donor leucocyte‐endothelial interactions and protects against acute graft‐versus‐host disease
样品类型: Plasma - 文献: Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function
样品类型: Serum,Cell culture supernatant - 文献: Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy
样品类型: Serum,Cell lysate,Tissue homogenate - 文献: Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis
样品类型: Tissue homogenate - 文献: Purification, characterization and bioactivities of polysaccharides from Pleurotus ferulae
样品类型: Cell culture supernatant - 文献: DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis
样品类型: serum,nasal lavage fluid - 文献: Effects of iron chelating agent on Schistosoma mansoni infected murine model
样品类型: Serum,Tissue homogenate - 文献: Components of heat-treated Helianthus annuus L. pectin inhibit tumor growth and promote immunity in a mouse CT26 tumor model
样品类型: Tissue homogenate - 文献: Macrophage Distribution Affected by Virus-Encoded Granulocyte Macrophage Colony Stimulating Factor Combined with Lactate Oxidase
样品类型: RAW264.7 cell - 文献: Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma
样品类型: RAW264.7 cell - 文献: Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway
样品类型: Serum - 文献: spp. in experimentally infected immunocompetent and immunosuppresed mice
样品类型: Serum,Tissue homogenate - 文献: The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis
样品类型: Tissue homogenate - 文献: Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IκB/P38 MAPK signaling pathway
样品类型: Tissue homogenate,Cell culture supernatant - 文献: Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway
样品类型: serum - 文献: Febuxostat Alleviates Allergic Rhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6.
样品类型: nasal mucosa tissue - 文献: Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection
样品类型: Serum,Cell culture supernatant - 文献: BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice
样品类型: BMDCs - 文献: Immunomodulatory effect of papaya (Carica papaya) pulp and seed extracts as a potential natural treatment for bacterial stress
样品类型: Serum - 文献: Preadipocytes In Vitro
样品类型: Cell culture supernatant - 文献: ROS Generated by Upconversion Nanoparticle-Mediated Photodynamic Therapy Induces Autophagy via PI3K/AKT/ mTOR Signaling Pathway in M1 Peritoneal Macrophage.
样品类型: peritoneal macrophages