MS Mouse MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit
MS-小鼠单核细胞趋化蛋白1(MCP-1)酶联免疫吸附测定试剂盒
规格: | / 96T / 48T / 96T*5 |
价格: | / ¥3080 / ¥2180 / ¥13860 |
货号:E-MSEL-M0012
反应性: 小鼠
检测范围: 15.63~1000 pg/mL
灵敏度: 9.38 pg/mL
MS系列
MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。
用途
该试剂盒用于体外定量检测小鼠serum, plasma and other biological fluids中MCP-1浓度
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗小鼠MCP-1抗体包被于酶标板上,实验时样品或标准品中的小鼠MCP-1会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠MCP-1抗体和辣根过氧化物酶标记的亲和素。抗小鼠MCP-1抗体与结合在包被抗体上的小鼠MCP-1结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,MCP-1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中MCP-1的浓度。
反应类型 | 夹心法 |
规格 | 96T |
反应时间 | 3.5h |
反应性 | 小鼠 |
检测方法 | Colormetric |
检测范围 | 15.63—1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 25μL |
样本类型 | serum, plasma and other biological fluids |
特异性
可检测样本中的小鼠MCP-1,且与其它相关蛋白无明显交叉反应。
重复性
板内,板间变异系数均<10%。
典型数据
由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。
(pg/mL) | O.D | Average | Corrected |
---|---|---|---|
1000 | 2.563 2.567 |
2.565 | 2.506 |
500 | 1.763 1.765 |
1.764 | 1.705 |
250 | 0.951 0.913 |
0.932 | 0.873 |
125 | 0.535 0.535 |
0.535 | 0.476 |
62.5 | 0.264 0.242 |
0.253 | 0.194 |
31.25 | 0.174 0.156 |
0.165 | 0.106 |
15.63 | 0.118 0.134 |
0.126 | 0.067 |
0 | 0.052 0.066 |
0.059 | -- |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) |
53.10 | 142.67 | 493.35 | 55.18 | 141.85 | 506.70 |
Standard
deviation |
3.69 | 8.12 | 25.85 | 3.82 | 8.51 | 21.18 |
C V (%) | 6.95 | 5.69 | 5.24 | 6.92 | 6.00 | 4.18 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum (n=5) | 94-108 | 99 |
EDTA plasma (n=5) | 86-101 | 93 |
Cell culture media (n=5) | 93-107 | 100 |
线性
分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
---|---|---|---|---|
1:2 | Range (%) | 95-111 | 94-111 | 87-101 |
Average (%) | 103 | 101 | 94 | |
1:4 | Range (%) | 91-108 | 96-110 | 87-100 |
Average (%) | 99 | 103 | 94 | |
1:8 | Range (%) | 93-109 | 97-109 | 90-104 |
Average (%) | 100 | 102 | 96 | |
1:16 | Range (%) | 96-109 | 92-106 | 86-98 |
Average (%) | 102 | 98 | 93 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。
中文名称 | 英文名称 | 规格 | 保存条件 |
---|---|---|---|
ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃,可存放6个月 |
冻干标准品 | Reference Standard | 2 支 | |
浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光),可存放6个月 |
标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
封板覆膜 | Plate Sealer | 5 张 | |
产品说明书 | Product Description | 1 份 | |
质检报告 | Certificate of Analysis | 1 份 |
说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。
试验所需自备物品
- 1. 酶标仪(450nm波长滤光片)
- 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3. 37℃恒温箱,双蒸水或去离子水
- 4. 吸水纸
操作步骤
|
1.对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟 |
2.弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟 | |
3.弃掉板内液体,洗板3次 | |
4.每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次 | |
5.每孔加入90μL底物溶液,37℃孵育15分钟 | |
6.每孔加入50μL终止液 | |
7.立即在450nm波长下读数,处理数据 |
客户发表文献
- 文献: Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
样品类型: B16–F10 cells,Red blood cells - 文献: Inflammation-Regulated Nanodrug Sensitizes Hepatocellular Carcinoma to Checkpoint Blockade Therapy by Reprogramming the Tumor Microenvironment
样品类型: Hepa 1-6 cells-xenograft - 文献: Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism
样品类型: bronchoalveolar fluid,RAW264.7 cell - 文献: MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy
样品类型: BALF - 文献: Biocompatible Assessment of Erythrocyte Membrane-Camouflaged Polymeric PLGA Nanoparticles in Pregnant Mice: Both on Maternal and Fetal/Juvenile Mice
样品类型: serum - 文献: RRP Regulates Autophagy through the AMPK Pathway to Alleviate the Effect of Cell Senescence on Atherosclerosis
样品类型: serum - 文献: Inositol Alleviates Pulmonary Fibrosis by Promoting Autophagy via Inhibiting the HIF-1α-SLUG Axis in Acute Respiratory Distress Syndrome
样品类型: serum,Human pulmonary alveolar epithelial cells (HPAEpi - 文献: A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols
样品类型: plasma - 文献: Hepatocyte-specific deficiency of Nrf2 exacerbates carbon tetrachloride-induced liver fibrosis via aggravated hepatocyte injury and subsequent inflammatory and fibrogenic responses
样品类型: Primary hepatocytes - 文献: Serum metabolomics analysis reveals amelioration effects of sea cucumber ether phospholipids on oxidative stress and inflammation in high-fat diet-fed mice
样品类型: serum - 文献: MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis
样品类型: serum - 文献: Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
样品类型: Plasma - 文献: Fenugreek attenuates obesity-induced inflammation and improves insulin resistance through downregulation of iRhom2/TACE
样品类型: Serum - 文献: Wu-tou decoction attenuates neuropathic pain via suppressing spinal astrocytic IL-1R1/TRAF6/JNK signaling
样品类型: Tissue homogenate - 文献: AMP-activated protein kinase reduces inflammatory responses and cellular senescence in pulmonary emphysema
样品类型: Bronchoalveolar Lavage Fluid - 文献: Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome
样品类型: plasma - 文献: Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice
样品类型: Serum - 文献: Anti-IFN-γ Antibody Promotes Osteoclastogenesis in Human Bone Marrow Monocyte-Derived Macrophages Co-Cultured with Tuberculosis-Activated Th1 Cells
样品类型: CD4+T helper 1 cells(Th1 cells),bone marrow monocyte/ma - 文献: Targeting PERK mediated endoplasmic reticulum stress attenuates neuroinflammation and alleviates lipopolysaccharide-induced depressive-like behavior in male mice
样品类型: plasma - 文献: Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
样品类型: serum - 文献: Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice
样品类型: serum - 文献: Mesenchymal Stem Cells Attenuate Acute Lung Injury in Mice Partly by Suppressing Alveolar Macrophage Activation in a PGE2-Dependent Manner
样品类型: MH-S cell - 文献: Limonin ameliorates acute pancreatitis by suppressing JAK2/STAT3 signaling pathway
样品类型: serum - 文献: The Microglial membrane receptor TREM2 mediates exosome secretion to promote phagocytosis of amyloid-β by microglia
样品类型: Bv2 cell - 文献: Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin
样品类型: Serum,Cell culture supernatant - 文献: The role of Dock2 on macrophage migration and functions during Citrobacter rodentium infection
样品类型: Colon tissue - 文献: Rosmanol and Carnosol Synergistically Alleviate Rheumatoid Arthritis through Inhibiting TLR4/NF-κB/MAPK Pathway
样品类型: serum - 文献: Seizure-induced neuroinflammation contributes to ectopic neurogenesis and aggressive behavior in pilocarpine-induced status epilepticus mice
样品类型: Cell lysate - 文献: N-acetyl-chitooligosaccharide attenuates inflammatory responses by suppression of NF-κB signaling, MAPK and NLRP3 inflammasome in macrophages
样品类型: Cell culture medium - 文献: Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells
样品类型: serum - 文献: The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice
样品类型: Serum - 文献: Major Outer Membrane Protein from Legionella pneumophila Inhibits Phagocytosis but Enhances Chemotaxis of RAW 264.7 Macrophages by Regulating the FOXO1/Coronin-1 Axis
样品类型: U87 cells - 文献: COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice
样品类型: Tissue homogenate - 文献: Rhamnetin ameliorates macrophage-mediated inflammation and pro-atherosclerosis pathways in apolipoprotein E-deficient mice
样品类型: aorta - 文献: Molecular mechanisms of the analgesic action of Wu-tou Decoction on neuropathic pain in mice revealed using microarray and network analysis
样品类型: Tissue homogenate - 文献: Diet-Derived Advanced Glycation End Products (dAGEs) Induce Proinflammatory Cytokine Expression in Cardiac and Renal Tissues of Experimental Mice: Protective Effect of Curcumin
样品类型: serum - 文献: The protective effects of Pimavanserin against cerebral ischemia-induced brain injury
样品类型: brain tissue - 文献: Dendritic cells enhance UHMWPE wear particle‐induced osteoclast differentiation of macrophages
样品类型: Cell culture supernatant - 文献: NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis
样品类型: Raw 264.7 cell - 文献: Effect of Intratracheal Instillation of ZnO Nanoparticles on Acute Lung Inflammation Induced by Lipopolysaccharides in Mice
样品类型: BALF - 文献: IGF2 inhibits hippocampal over-activated microglia and alleviates depression-like behavior in LPS- treated male mice
样品类型: Serum - 文献: IL-17 upregulates MCP-1 expression via Act1 / TRAF6 / TAK1 in experimental autoimmune myocarditis
样品类型: serum - 文献: Inhibition of LOX-1 prevents inflammation and photoreceptor cell death in retinal degeneration
样品类型: Cell culture supernatant - 文献: Bee venom attenuates neurodegeneration and motor impairment and modulates the response to L-dopa or rasagiline in a mice model of Parkinson’s disease
样品类型: Tissue homogenate - 文献: Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet
样品类型: Serum - 文献: 4-Hydroxyisoleucine relieves inflammation through iRhom2-dependent pathway in co-cultured macrophages and adipocytes with LPS stimulation
样品类型: Cell sample - 文献: Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway
样品类型: serum - 文献: Peroxisome proliferator-activated receptor-γ agonist pioglitazone fails to attenuate renal fibrosis caused by unilateral ureteral obstruction in mice
样品类型: Tissue homogenate