Myeloperoxidase (MPO) Activity Assay Kit
髓过氧化物酶(MPO)比色法测试盒
| 规格： | / 48T(24 samples) / 96T(48 samples) |
| 价格： | / ￥320 / ￥560 |
货号：E-BC-K074-M
检测仪器及波长: 酶标仪(450-470 nm，最佳检测波长460 nm)
样本类型: 血清;血浆;乳汁;动物组织样本
检测原理
髓过氧化物酶将过氧化氢还原成一种复合物，该复合物通过供氢体邻联茴香胺供氢后生成黄色产物，在460 nm处通过比色法测定产物的生成量，从而计算出MPO的活力。
关键信息
| 指标简称 | MPO |
| 样本类型 | 血清;血浆;乳汁;动物组织样本 |
| 灵敏度 | 19.42 U/L |
| 检测范围 | 19.42-893.31 U/L |
| 精密度 | 批间差：7.3%批内差：5.4% |
| 检测仪器及波长 | 酶标仪 |
| 特殊仪器 | 恒温水浴锅（60℃） |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择预期差异较大的2-3个样本，稀释成不同浓度进行预实验，结合检测范围：19.42 -893.31 U/L，不同样本稀释比例如下表(仅供参考)：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
大鼠血清 |
不稀释 |
|
人血浆 |
不稀释 |
小鼠血浆 |
不稀释 |
|
人乳汁 |
不稀释 |
5%大鼠肾脏组织 |
不稀释 |
|
细胞上清 |
不稀释 |
5%大鼠脾脏组织 |
不稀释 |
注：稀释液为试剂二应用液
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