Nitric Oxide (NO) Colorimetric Assay Kit
一氧化氮(NO)比色法测试盒
规格: | / 50 assays(48 samples) / 100 assays(96 samples) |
价格: | / ¥240 / ¥380 |
货号:E-BC-K035-S
检测仪器及波长: 紫外-可见光分光光度计(550nm)
样本类型: 血清;血浆及动植物组织样本
检测原理
NO在体内或水溶液中极易氧化生成NO2-,与显色剂生成淡红色偶氮化合物(如下图),生成偶氮化合物的浓度与NO的浓度具有线性关系,通过比色可以间接计算NO的浓度。试剂一和试剂二的作用,为除去样本有色物质的干扰。
关键信息
指标简称 | NO |
样本类型 | 血清;血浆及动植物组织样本 |
灵敏度 | 0.97 μmol/L |
检测范围 | 0.97-700 μmol/L |
精密度 | 批间差:5.2%批内差:3.4% |
检测仪器及波长 | 分光光度计 |
保存条件 | Reagent 6 -20℃,其余2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3个预期差异大的样本稀释成不同浓度进行预实验,结合本试剂盒的线性范围(0.97-700 μmol/L),选取最佳稀释倍数,进行正式批量实验。不同样本的稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
不稀释 |
大鼠血清 |
不稀释 |
人血浆 |
不稀释 |
大鼠血浆 |
不稀释 |
10%小鼠肝匀浆 |
不稀释 |
10%绿萝匀浆 |
不稀释 |
注:稀释液为双蒸水、生理盐水(0.9% NaCl)或PBS(0.01 M,pH 7.4)。
相关产品
货号 | 产品名称 |
---|---|
E-BC-K070-S | 亚硝酸盐比色法测试盒 |
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