Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit
游离脂肪酸(NEFA/FFA) 比色法测试盒
| 规格： | / 50 assays(25 samples) / 100 assays(50 samples) |
| 价格： | / ￥380 / ￥680 |
货号：E-BC-K013-S
检测仪器及波长: 紫外-可见光分光光度计（715 nm）
样本类型: 血清;动物组织
检测原理
在弱酸性条件下，游离脂肪酸与铜盐反应生成铜皂，在715 nm处有特征吸收峰，在一定范围内游离脂肪酸含量与显色程度呈线性关系，可计算出游离脂肪酸的含量。
关键信息
| 指标简称 | NEFA/FFA |
| 样本类型 | 血清;动物组织 |
| 灵敏度 | 0.05 mmol/L |
| 检测范围 | 0.05-2.0 mmol/L |
| 精密度 | 批间差：6.1%批内差：2.2% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围（0.05-2 mmol/L），请参考下表稀释：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
大鼠肝组织匀浆抽提液 |
1-3 |
大鼠血清 |
不稀释 |
|
大鼠心组织匀浆抽提液 |
不稀释 |
小鼠血清 |
不稀释 |
|
大鼠肾组织匀浆抽提液 |
不稀释 |
人血清 |
不稀释 |
|
小鼠肝组织匀浆抽提液 |
1-3 |
|
|
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