OT(Oxytocin) ELISA Kit
催产素(OT)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2600 / ￥1800 / ￥11050 |
货号：E-EL-0029
反应性: 通用
检测范围: 15.63~1000 pg/mL
灵敏度: 9.38 pg/mL
用途
该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中OT浓度。
检测原理
本试剂盒采用竞争ELISA法。用OT抗原包被于酶标板上，实验时样品或标准品中的OT与包被的OT竞争生物素标记的抗OT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，OT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中OT的浓度。
| 反应类型 | 竞争法 |
| 规格 | 96T |
| 反应时间 | 2.0h |
| 反应性 | 通用 |
| 检测方法 | Colormetric |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50μL |
| 样本类型 | 血清、血浆或其他相关生物液体 |
特异性
可检测样本中的OT，且与其它相关蛋白无明显交叉反应。
重复性
板内，板间变异系数均<10%。
典型数据
由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | O.D | Average |
|---|---|---|
| 1000 | 0.342 0.348 |
0.345 |
| 500 | 0.43 0.446 |
0.438 |
| 250 | 0.625 0.597 |
0.611 |
| 125 | 0.885 0.923 |
0.904 |
| 62.5 | 1.325 1.307 |
1.316 |
| 31.25 | 1.784 1.762 |
1.773 |
| 15.63 | 2.152 2.168 |
2.16 |
| 0 | 2.72 2.73 |
2.725 |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) |
53.20 | 153.88 | 495.39 | 56.27 | 139.06 | 544.58 |
| Standard
deviation |
2.78 | 7.02 | 16.10 | 2.97 | 6.04 | 23.85 |
| C V (%) | 5.23 | 4.56 | 3.25 | 5.28 | 4.34 | 4.38 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 92-103 | 98 |
| EDTA plasma (n=5) | 92-104 | 99 |
| Cell culture media (n=5) | 88-102 | 94 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 94-110 | 93-107 | |
| Average (%) | 92 | 101 | 100 | |
| 1:4 | Range (%) | 88-100 | 91-108 | 94-108 |
| Average (%) | 94 | 99 | 101 | |
| 1:8 | Range (%) | 80-92 | 92-106 | 98-112 |
| Average (%) | 87 | 97 | 103 | |
| 1:16 | Range (%) | 90-103 | 97-110 | 96-108 |
| Average (%) | 95 | 102 | 101 | |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周；如果一周以后才使用试剂盒，请拆开试剂盒按照下表中的条件分别保存各组分。
| 中文名称 | 英文名称 | 规格 | 保存条件 |
|---|---|---|---|
| ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃，可存放6个月 |
| 冻干标准品 | Reference Standard | 2支 | |
| 浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
| 浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光)，可存放6个月 |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
| 生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
| 底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
| 反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
- 1.酶标仪(450nm波长滤光片)
- 2.高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3.37℃恒温箱，双蒸水或去离子水
- 4.吸水纸
操作步骤
|
|
1. 对应板孔中加入50μL标准品工作液或样本后，立即每孔加入50μL生物素化抗体工作液，37℃孵育90分钟 |
| 2. 弃掉板内液体，洗板3次 | |
| 3. 每孔加入100μL HRP酶结合物工作液，37℃孵育30分钟，弃掉板内液体，洗板5次 | |
| 4. 每孔加入90μL底物溶液，37℃孵育15分钟 | |
| 5. 每孔加入50μL终止液 | |
| 6. 立即在450nm波长下读数，处理数据 |
客户发表文献
- 文献: Oxytocin Nanogels Inhibit Innate Inflammatory Response for Early Intervention in Alzheimer’s Disease
样品类型: brain - 文献: Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial
样品类型: serum - 文献: Protective effect of oxytocin on LPS-induced acute lung injury in mice
样品类型: Tissue homogenate - 文献: Social isolation rearing-induced anxiety and response to agomelatine in male and female rats: Role of corticosterone, oxytocin, and vasopressin:
样品类型: Plasma - 文献: Protective Effects of Intranasally Administrated Oxytocin-Loaded Nanoparticles on Pentylenetetrazole-Kindling Epilepsy in Terms of Seizure Severity, Memory, Neurogenesis, and Neuronal Damage
样品类型: serum - 文献: The effects of acute foot shock stress on empathy levels in rats
样品类型: Serum,Tissue homogenate - 文献: Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring
样品类型: serum - 文献: Atrial natriuretic peptide and posterior pituitary neurohormone changes in patients with acute schizophrenia
样品类型: Serum - 文献: Targeting the oxytocin system to ameliorate early life depressive-like behaviors in maternally-separated rats
样品类型: plasma - 文献: The Relationship Between the Oxytocin Level and Rejection Sensitivity, Childhood Traumas, and Attachment Styles in Borderline Personality Disorder
样品类型: Serum - 文献: Acetaminophen (paracetamol) affects empathy-like behavior in rats: Dose-response relationship
样品类型: Serum,Tissue homogenate - 文献: Regular Aerobic Voluntary Exercise Increased Oxytocin in Female Mice: The Cause of Decreased Anxiety and Increased Empathy-Like Behaviors
样品类型: blood,brain - 文献: Immediate breastfeeding and skin-to-skin contact during cesarean section decreases maternal oxidative stress, a prospective randomized case-controlled study
样品类型: Serum - 文献: Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance
样品类型: Serum - 文献: Variability of serum reproductive hormones in cows presenting various reproductive conditions in semi-arid areas of the North West Province, South Africa
样品类型: Serum - 文献: The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial
样品类型: serum - 文献: The Effect of Oxytocin and an Enriched Environment on Anxiety-Like Behaviour and Corticosterone Levels in a Prenatally Stressed Febrile Seizure Rat Model
样品类型: Hypothalamic tissue - 文献: Prolonged efavirenz exposure reduces peripheral oxytocin and vasopressin comparable to known drugs of addiction in male Sprague Dawley rats
样品类型: plasma - 文献: Evaluation of behavior and endocrinological changes after REM sleep deprivation-induced mania-like behavior in mice
样品类型: serum
