Total Bilirubin (TBIL) Colorimetric Assay Kit
总胆红素(TBIL)比色法测试盒
规格: | / 48T(22 samples) / 96T(46 samples) |
价格: | / ¥280 / ¥380 |
货号:E-BC-K760-M
检测仪器及波长: 酶标仪(565 nm)
样本类型: 动物血清样本
检测原理
在加速剂作用下 ,间接胆红素内氢键被破坏,使非水溶性的间接胆红素与直接胆红素在酸性条件下与偶氮试剂反应生成偶氮胆红素,生成的偶氮胆红素在565 nm处有最大吸收,测定吸光度变化可求出血清中总胆红素含量 。
关键信息
指标简称 | TBIL |
样本类型 | 动物血清样本 |
灵敏度 | 0.7 μmol/L |
检测范围 | 0.7-50 μmol/L |
精密度 | 批间差:4%批内差:2.8% |
检测仪器及波长 | 酶标仪 |
保存条件 | 2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择预期差异较大的2-3个样本,稀释成不同浓度进行预实验,根据预实验结果,结合本试剂盒检测范围0.7-50 μmol/L,请参考下表稀释(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
不稀释 |
兔血清 |
不稀释 |
大鼠血清 |
不稀释 |
鸡血清 |
不稀释 |
小鼠血清 |
不稀释 |
猪血清 |
不稀释 |
注:样本稀释液为生理盐水(0.9% NaCl)。
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