Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)
总胆固醇(TC)比色法测试盒(单试剂COD-PAP法)
| 规格： | / 50 assays(48 samples) / 100 assays(96 samples) |
| 价格： | / ￥260 / ￥360 |
货号：E-BC-K109-S
检测仪器及波长: 紫外-可见光分光光度计（510 nm）
样本类型: 血清（浆）;组织
检测原理
总胆固醇(Total Cholesterol，TC)包括游离胆固醇和胆固醇酯。胆固醇酯可被胆固醇酯酶（cholesterol esterase，CE）水解成胆固醇和游离脂肪酸，胆固醇在胆固醇氧化酶（cholesterol oxidase，CO）的氧化作用下生成△4-胆甾烯酮和过氧化氢。过氧化氢在4-氨基安替吡啉和酚存在时，经过氧化物酶（peroxidase，POD）催化，反应生成苯醌亚胺非那腙的红色醌类化合物，其颜色深浅与TC含量成正比。
关键信息
| 指标简称 | TC |
| 样本类型 | 血清（浆）;组织 |
| 灵敏度 | 0.09 mmol/L |
| 检测范围 | 0.09-25.85 mmol/L |
| 精密度 | 批间差：2.8%批内差：1.1% |
| 检测仪器及波长 | 分光光度计 |
| 自备试剂 | 无水乙醇 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的检测范围（0.09-25.85 mmol/L），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
10%小鼠肝匀浆 |
不稀释 |
|
小鼠血清 |
不稀释 |
10%小鼠肾匀浆 |
不稀释 |
|
大鼠血浆 |
不稀释 |
10%小鼠心匀浆 |
不稀释 |
|
|
|
|
注：血清（浆）样本的稀释液为生理盐水（0.9% NaCl）或PBS（0.01M，pH 7.4），动物组织样本的稀释液为无水乙醇。
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