Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)
甘油三酯(TG)比色法测试盒(单试剂GPO-PAP法)
| 规格： | / 96T(92 samples) |
| 价格： | / ￥320 |
货号：E-BC-K261-M
检测仪器及波长: 酶标仪（490 -525 nm，最佳波长510 nm）
样本类型: 血清（浆）;动物组织
检测原理
甘油三酯(Triglycerides，TG)可被脂蛋白脂酶（Lipoprotein Lipase）水解甘油和游离脂肪酸，甘油在甘油激酶（glycerol kinase，GK）的作用下甘油-3-磷酸和ADP。甘油-3-磷酸在3-磷酸甘油氧化酶（glycerol phosphate oxidase，GPO）作用下产生过氧化氢，过氧化氢在4-氨基安替吡啉和酚存在时，经过氧化物酶（peroxidase，POD）催化，反应生成苯醌亚胺非那腙的红色醌类化合物，其颜色深浅与TG含量成正比。
关键信息
| 指标简称 | TG |
| 样本类型 | 血清（浆）;动物组织 |
| 灵敏度 | 0.14 mmol/L |
| 检测范围 | 0.14-10 mmol/L |
| 精密度 | 批间差：9.2%批内差：4.1% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3例预期差异大的样本稀释成不同浓度进行预实验，不同样本稀释倍数请参考下表：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
10%小鼠肝匀浆 |
不稀释 |
|
小鼠血清 |
不稀释 |
10%小鼠肾匀浆 |
不稀释 |
|
大鼠血浆 |
不稀释 |
10%小鼠心匀浆 |
不稀释 |
|
|
|
|
|
注：血清（浆）样本的稀释液为生理盐水（0.9% NaCl）或PBS（0.01M，pH 7.4），动物组织样本的稀释液为无水乙醇。
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