Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)
尿素(BUN)比色法测试盒 (二乙酰肟法)
| 规格： | / 50 assays(48 samples) / 100 assays(96 samples) |
| 价格： | / ￥200 / ￥320 |
货号：E-BC-K329-S
检测仪器及波长: 紫外-可见光分光光度计（520 nm）
样本类型: 血清（浆）;唾液;尿液
检测原理
尿素与二乙酰在酸性反应环境中加热缩合成色素原二嗪化合物，其显色的深浅与尿素含量成正比，由于二乙酰不稳定，故通常在反应体系中先由二乙酰一肟与强酸作用，产生二乙酰，再与尿素反应，缩合成红色的二嗪化合物。
关键信息
| 指标简称 | BUN,UN |
| 样本类型 | 血清（浆）;唾液;尿液 |
| 灵敏度 | 0.12 mmol/L |
| 检测范围 | 0.12-15 mmol/L |
| 精密度 | 批间差：9.9%批内差：4.9% |
| 检测仪器及波长 | 分光光度计 |
| 特殊仪器 | 100℃恒温水浴锅 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的线性范围（0.12-15 mmol/L），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人尿液 |
45-60 |
小鼠血清 |
不稀释 |
|
人血浆 |
不稀释 |
大鼠尿液 |
45-60 |
|
人血清 |
不稀释 |
兔血浆 |
不稀释 |
|
人唾液 |
不稀释 |
|
|
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-K183-S | 尿素(BUN)比色法测试盒(脲酶法) |
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