Urea (BUN) Colorimetric Assay Kit (Urease Method)
尿素(BUN)比色法测试盒 (脲酶法)
| 规格： | / 96T(40 samples) |
| 价格： | / ￥320 |
货号：E-BC-K183-M
检测仪器及波长: 酶标仪（565-595 nm，最佳波长580 nm）
样本类型: 血清（浆）;尿液;唾液;乳汁
检测原理
尿素在脲酶的作用下分解产生铵离子和二氧化碳，铵离子在碱性介质中与酚衍生物生成绿色的物质，该物质的生成量与尿素含量呈正比。
关键信息
| 指标简称 | BUN,UN |
| 样本类型 | 血清（浆）;尿液;唾液;乳汁 |
| 灵敏度 | 0.09 mmol/L |
| 检测范围 | 0.28-35 mmol/L |
| 精密度 | 批间差：4.3%批内差：2.8% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 3个月 |
样本稀释倍数
在正式检测前，需选择2-3例预期差异大的样本稀释成不同浓度进行预实验，不同样本的稀释倍数请参考下表：
|
样本名称 |
稀释倍数 |
样本名称 |
稀释倍数 |
|
人血清 |
不稀释 |
大鼠血浆 |
不稀释 |
|
人唾液 |
不稀释 |
人尿液 |
50-70 |
注：稀释液为PBS（0.01 M，pH 7.4）或生理盐水（0.9% NaCl）。
相关产品
客户发表文献
- 文献: Cyclic reactions-mediated self-supply of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy
样品类型: MDA-MB-231 cells - 文献: An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo
样品类型: serum - 文献: A solid lipid coated calcium peroxide nanocarrier enables combined cancer chemo/chemodynamic therapy with O2/H2O2 self-sufficiency
样品类型: Serum,Plasma - 文献: Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats
样品类型: plasma,urine - 文献: Ultrasonically Fabricated Beta-Carotene Nanoemulsion: Optimization, Characterization and Evaluation of Combinatorial Effect with Quercetin on Streptozotocin-Induced Diabetic Rat Model
样品类型: serum - 文献: Capsaicin Prevents Contrast-Associated Acute Kidney Injury through Activation of Nrf2 in Mice
样品类型: serum - 文献: Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome
样品类型: plasma - 文献: Yishen Tongluo formula alleviates diabetic kidney disease through regulating Sirt6/TGF-β1/Smad2/3 pathway and promoting degradation of TGF-β1
样品类型: kidney - 文献: Stewed Rhubarb Decoction Ameliorates Adenine-Induced Chronic Renal Failure in Mice by Regulating Gut Microbiota Dysbiosis.
样品类型: plasma - 文献: Fabrication and Evaluation of Quercetin Nanoemulsion: A Delivery System with Improved Bioavailability and Therapeutic Efficacy in Diabetes Mellitus
样品类型: serum - 文献: Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways
样品类型: serum - 文献: Potential Protective Effects of Ruta Chalepensis L. Extracts Against Gentamicin-Induced Nephrotoxicity via Reduction on Apoptotic, DNA Damage and Oxidative Stress Markers in Mice
样品类型: serum - 文献: Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells
样品类型: serum - 文献: Relative comparison of chronic kidney disease-mineral and bone disorder rat models
样品类型: Serum - 文献: Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides
样品类型: Plasma - 文献: Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners
样品类型: plasma - 文献: Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis
样品类型: serum - 文献: Toxic effects of subchronic oral acetamiprid exposure in rats:
样品类型: plasma
