Vitamin C (VC) Colorimetric Assay Kit
维生素C(VC)比色法测试盒
| 规格： | / 50 assays(48 samples) / 100 assays(96 samples) |
| 价格： | / ￥320 / ￥580 |
货号：E-BC-K034-S
检测仪器及波长: 紫外-可见光分光光度计（533 nm）
样本类型: 血清（浆）;动植物组织
检测原理
VC在菲啰啉的存在下，可使三价铁离子还原成二价铁离子，后者在特定的环境下可与菲啰啉形成粉红色复合物，在536 nm处有最大吸收波长。通过比色，可计算出VC的含量。
关键信息
| 指标简称 | VC |
| 样本类型 | 血清（浆）;动植物组织 |
| 灵敏度 | 0.35 μg/mL |
| 检测范围 | 0.35-20 μg/mL |
| 精密度 | 批间差：3.3%批内差：2.3% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
本试剂盒的检测范围在0.27-155.4 U/mL，测试前选择2-3个预期差异大的样本进行预实验以确定最佳取样浓度。不同样本稀释比例范围如下表（仅供参考）：
|
样本 |
稀释倍数 |
上样量 |
|
10%大鼠肝匀浆 |
25-50 |
50 μL |
|
10%大鼠肾匀浆 |
10-25 |
50 μL |
|
10%大鼠脑匀浆 |
5-10 |
50 μL |
|
人血清 |
不稀释 |
100 μL |
|
293T细胞上清 |
不稀释 |
100 μL |
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-K033-S | 维生素E(VE)比色法测试盒 |
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