(KO Validated) Vimentin Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1580 / ￥2520 / ￥4020 |
货号：E-AB-63601
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC,IF
Overview
| Synonyms | CTRCT30,HEL113,Vimentin,VIM,vimentin,Vimentin抗体 |
| Swissprot | P08670 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant fusion protein of human Vimentin (NP_003371.2). |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,, 1:500-1:2000;IHC,, 1:50-1:200;IF,, 1:50-1:200; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Background | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. |
Images
Western blot analysis of extracts of various cell lines using Vimentin Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human tonsil using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
