AKT Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3580 |
货号：E-AB-63467
宿主: Rabbit
反应性: H,M,R
应用: IHC,IF
Overview
| Synonyms | AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,AKT1,AKT1抗体 |
| Swissprot | P31749 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human AKT (NP_005154.2). |
| Application | IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | IHC,, 1:50-1:100;IF,, 1:50-1:100; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. |
Images
Immunohistochemistry of paraffin-embedded Human mammary cancer using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human uterine cancer using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human tonsil using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using AKT Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
