Annexin V-APC/7-AAD Apoptosis Kit
Annexin V-APC / 7-AAD细胞凋亡检测试剂盒
规格: | / 20Assays / 50Assays / 100Assays / 200Assays |
价格: | / ¥850 / ¥1350 / ¥2100 / ¥3100 |
货号:E-CK-A218
检测原理
Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。 细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。
基本信息
应用类型 | 细胞凋亡-Annexin V |
检测方法 | 荧光 |
样本类型 | 细胞 |
检测时间 | 40min |
检测仪器 | 流式细胞仪 |
荧光 | APC |
Ex/Em (nm) | 650/660 |
Channel set | APC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 2~8°C, shading light. |
运输条件 | 冰袋 |
保质期 | 12 months |
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