Annexin V-APC/PI Apoptosis Kit
Annexin V-APC / PI细胞凋亡检测试剂盒
规格: | / 20Assays / 50Assays / 100Assays / 200Assays |
价格: | / ¥750 / ¥1250 / ¥2000 / ¥3000 |
货号:E-CK-A217
检测原理
Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。 细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。
基本信息
应用类型 | 细胞凋亡-Annexin V |
检测方法 | 荧光 |
样本类型 | 细胞 |
检测时间 | 40min |
检测仪器 | 流式细胞仪 |
荧光 | APC |
Ex/Em (nm) | 650/660 |
Channel set | APC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 2~8°C, shading light |
运输条件 | 冰袋 |
保质期 | 12 months |
客户发表文献
- 文献: Pyroptosis Remodeling Tumor Microenvironment to Enhance Pancreatic Cancer Immunotherapy Driven by Membrane Anchoring Photosensitizer
- 文献: Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer
- 文献: Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways
- 文献: Effective Delivery of siRNA-Loaded Nanoparticles for Overcoming Oxaliplatin Resistance in Colorectal Cancer.
- 文献: TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB
- 文献: Analysis of the Roles of the ISLR2 Gene in Regulating the Toxicity of Zearalenone Exposure in Porcine Intestinal Epithelial Cells
- 文献: Calycosin-7-glucoside promotes mitochondria-mediated apoptosis in hepatocellular carcinoma by targeting thioredoxin 1 to regulate oxidative stress
- 文献: Circular RNA SMO sponges miR-338-3p to promote the growth of glioma by enhancing the expression of SMO
- 文献: Lactobacillus Protects Against S. Typhimurium–Induced Intestinal Inflammation by Determining the Fate of Epithelial Proliferation and Differentiation
样品类型: Cell sample - 文献: Knockdown of enhancer of rudimentary homolog inhibits proliferation and metastasis in ovarian cancer by regulating epithelial-mesenchymal transition
样品类型: Cell culture supernatant - 文献: On-Demand Release of Fucoidan from a Multilayered Nanofiber Patch for the Killing of Oral Squamous Cancer Cells and Promotion of Epithelial Regeneration
- 文献: PARP inhibitors intervene DNA damage repair for the enhancement of tumor photodynamic therapy
- 文献: SOX4-activated lncRNA MCM3AP-AS1 aggravates osteoarthritis progression by modulating miR-149-5p/Notch1 signaling
- 文献: Curcumin-loaded zein/pectin nanoparticles: Caco-2 cellular uptake and the effects on cell cycle arrest and apoptosis of human hepatoma cells (HepG2)
- 文献: Silencing long non-coding RNAs nicotinamide nucleotide transhydrogenase antisense RNA 1 inhibited papillary thyroid cancer cell proliferation, migration and invasion and promoted apoptosis via targeting miR-199a-5p
样品类型: Cell(TPC-1 or IHH-4 cell) - 文献: Down-regulation of Xist and Mir-7a-5p improves LPS-induced myocardial injury
样品类型: Cell sample - 文献: NFIC promotes the vitality and osteogenic differentiation of rat dental follicle cells
- 文献: FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer:
- 文献: CD44 enhances adriamycin resistance in chronic myelogenous leukaemia cells K562
- 文献: Trifluoperazine Synergistically Potentiates Bortezomib-Induced Anti-Cancer Effect in Multiple Myeloma via Inhibiting P38 MAPK/NUPR1