Annexin V-FITC/7-AAD Apoptosis Kit
Annexin V-FITC / 7-AAD细胞凋亡检测试剂盒
| 规格： | / 20Assays / 50Assays / 100Assays / 200Assays |
| 价格： | / ￥500 / ￥1000 / ￥1600 / ￥2560 |
货号：E-CK-A212
检测原理
Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。 细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，细胞核染料（DAPI、PI、7-AAD）可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。
基本信息
| 应用类型 | 细胞凋亡-Annexin V |
| 检测方法 | 荧光 |
| 样本类型 | 细胞 |
| 检测时间 | 40min |
| 检测仪器 | 流式细胞仪 |
| 荧光 | FITC |
| Ex/Em (nm) | 490/530 |
| Channel set | FITC |
| 自备试剂 | PBS(E-BC-R187), 去离子水 |
| 保存温度 | 2~8°C, shading light. |
| 运输条件 | 冰袋 |
| 保质期 | 12 months |
操作指南
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