BAX Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22128
宿主: Mouse
反应性: H,M,R
应用: WB,IHC-p
Overview
| Synonyms | Apoptosis regulator BAX,BAX,Bax-protein,BAX,BAXA,Baxdelta2G9,Baxdelta2G9omega,Baxdelta2omega,Bcl-2-like protein 4,BCL2 associated X protein,BCL2 associated X protein omega,BCL2 associated X protein transcript variant delta2,Bcl2-L-4,BCL2L4,membrane isoform alpha,Bax Mouse (6F11)抗体 |
| Swissprot | Q07812 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of Bax |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:1000-2000;IHC,,1:100-200; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. |
| Tissue specificity | Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. |
| Clone No | Clone:1D8 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. |
Images
Western Blot analysis of 1) Hela, 2) C2C12, 3) PC-12 cells using Bax Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using Bax Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Mouse kidney tissue using Bax Monoclonal Antibody at dilution of 1:200.
客户发表文献
- 文献: The pivotal role of miRNA-21 in myocardial metabolic flexibility in response to short- and long-term high glucose treatment: Evidence in human cardiomyocyte cell line
样品类型: AC16 cell - 文献: Royal jelly protects brain tissue against fluoride-induced damage by activating Bcl-2/NF-κB/caspase-3/caspase-6/Bax and Erk signaling pathways in rats
样品类型: Brain tissue - 文献: The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines
样品类型: Cell culture supernatant
