CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
分型超氧化物歧化酶(CuZn-SOD/Mn-SOD)测试盒(羟胺法)
规格: | / 48T(44 samples) / 96T(92 samples) |
价格: | / ¥380 / ¥660 |
货号:E-BC-K022-M
检测仪器及波长: 酶标仪(530-570 nm,最适检测波长550 nm)
样本类型: 血清(浆);尿液;细胞及细胞上清;各种动植物组织
检测原理
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基(O2.-),它氧化羟胺形成亚硝酸盐,在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后,催化超氧阴离子自由基发生歧化反应,使形成的亚硝酸盐减少,进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值,来计算SOD的活性。同时,经过试剂处理的样本导致Mn-SOD失活,而不影响CuZn-SOD。
关键信息
指标简称 | CuZn-SOD,Mn-SOD,T-SOD |
样本类型 | 血清(浆);尿液;细胞及细胞上清;各种动植物组织 |
灵敏度 | 1.35 U/mL |
检测范围 | 1.35-62 U/mL |
精密度 | 批间差:9.6%批内差:5.1% |
检测仪器及波长 | 酶标仪 |
保存条件 | Reagent 4 -20℃,其余2-8℃ |
有效期 | 6个月 |
样本稀释倍数
在正式检测前,需选择2-3个预期差异较大的样本稀释成不同浓度,按操作表进行预实验,以确定最佳稀释倍数。不同样本稀释如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
小鼠血清 |
5-10倍 |
HepG2细胞(5.21mgprot/mL) |
15-25倍 |
大鼠血清 |
6-15倍 |
10%小鼠肝匀浆 |
100-200倍 |
尿液 |
2-3倍 |
10%小鼠脑匀浆 |
20-30倍 |
人胸水 |
2-3倍 |
10%小鼠肾匀浆 |
50-120倍 |
细胞上清 |
不稀释 |
10%大鼠肾匀浆 |
50-120倍 |
注:稀释液为生理盐水(0.9%NaCl)。
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