CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
分型超氧化物歧化酶(CuZn-SOD/Mn-SOD)测试盒(羟胺法)
| 规格： | / 50 Assays(25 samples) / 100 Assays(50 samples) |
| 价格： | / ￥360 / ￥580 |
货号：E-BC-K022-S
检测仪器及波长: 紫外-可见光分光光度计（550 nm）
样本类型: 血清（浆）;尿液;细胞及细胞上清;各种动植物组织
检测原理
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基（O2.-），它氧化羟胺形成亚硝酸盐，在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后，催化超氧阴离子自由基发生歧化反应，使形成的亚硝酸盐减少，进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值，来计算SOD的活性。高等生物细胞内含有有铜锌-SOD（CuZn-SOD）和锰-SOD（Mn-SOD）同时，经样本前处理过程的样本中Mn-SOD活力丧失，但CuZn-SOD活力不变。
关键信息
| 指标简称 | CuZn-SOD,T-SOD |
| 样本类型 | 血清（浆）;尿液;细胞及细胞上清;各种动植物组织 |
| 灵敏度 | 2.03 U/mL |
| 检测范围 | 2.03-155 U/mL |
| 精密度 | 批间差：7.2%批内差：4% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | Reagent 4 -20℃，其余2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异较大的样本设置不同的取样量，按操作表进行预实验，以确定最佳取样量。不同样本稀释如下表（仅供参考）：
|
样本 |
稀释倍数 |
上样量（μL） |
|
小鼠血清 |
3-5倍 |
20-30 |
|
人血清 |
不稀释 |
30-40 |
|
大鼠血清 |
3-5倍 |
20-30 |
|
大鼠血浆 |
1-2倍 |
20-30 |
|
人胸水 |
不稀释 |
30-50 |
|
人尿液 |
不稀释 |
30-50 |
|
10%小鼠肝匀浆 |
50-80倍 |
20-30 |
|
10%小鼠脑匀浆 |
8-12倍 |
20-30 |
|
10%小鼠肾匀浆 |
10-20 |
20-30 |
|
HepG2细胞（3.27 mgprot/mL） |
5-10倍 |
20-30 |
注：稀释液为生理盐水（0.9%NaCl）。
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