D-Lactic Acid/Lactate(LA)Colorimetric Assay Kit
D-乳酸(LA)比色法测试盒
| 规格： | / 48T(32 samples) / 96T(80 samples) |
| 价格： | / ￥1600 / ￥2800 |
货号：E-BC-K002-M
检测仪器及波长: 酶标仪（520-550 nm，最佳检测波长530 nm）
样本类型: 动物组织样本及血清（浆）;唾液等液体样本
检测原理
以氧化型辅酶Ⅰ（NAD+）为氢受体，D-乳酸脱氢酶（LDH）催化D-乳酸脱氢产生丙酮酸，使NAD+转化为还原型辅酶Ⅰ（NADH）。其中N-甲基吩嗪甲基硫酸盐（PMS）递氢使氯化硝基四氮唑蓝（NBT）还原为紫色呈色物，呈色物的吸光度在530 nm时与乳酸含量呈线性关系。整个过程中，L-乳酸不影响测定结果，因此，通过测定波长530 nm处吸光度值的变化，即可计算出样本中D-乳酸的含量。
关键信息
| 指标简称 | D-LA |
| 样本类型 | 动物组织样本及血清（浆）;唾液等液体样本 |
| 灵敏度 | 0.06 mmol/L |
| 检测范围 | 0.06-8.0 mmol/L |
| 精密度 | 批间差：7.7%批内差：3.8% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围（0.06-8.0 mmol/L），请参考下表稀释:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
小鼠血清 |
不稀释 |
|
人血浆 |
不稀释 |
兔血清 |
不稀释 |
|
大鼠血清 |
不稀释 |
10%大鼠肾组织 |
2-3 |
|
大鼠血浆 |
不稀释 |
10%大鼠脑组织 |
不稀释 |
注：稀释液为生理盐水或PBS（0.01 M，pH 7.4）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-K044-M | L-乳酸(LA)比色法测试盒 |
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