HSP27 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-31748
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC-p,IF,ELISA
Overview
| Synonyms | Heat shock 27kDa protein,28 kDa heat shock protein,CMT2F,DKFZp586P1322,epididymis secretory protein Li 102,Estrogen regulated 24 kDa protein,Estrogen-regulated 24 kDa protein,Heat shock 25kDa protein 1,Heat shock 27 kDa protein,Heat shock 27kD protein 1,Heat shock 27kDa protein 1,Heat shock 28kDa protein 1,Heat Shock Protein 27,Heat shock protein beta 1,Heat shock protein beta-1,heat shock protein family B (small) member 1,HEL-S-102,HMN2B,HS.76067,Hsp 25,HSP 27,Hsp 28,Hsp B1,Hsp25,HSP27,Hsp28,HspB1,HSPB1,SRP27,Stress responsive protein 27,Stress-responsive protein 27,HSP27抗体 |
| Swissprot | P04792 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human HSP27 around the non-phosphorylation site of Ser15. |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF,ELISA |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:300;IF,,1:50-1:200;ELISA,,1:10000-1:20000; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Cytoplasm>cytoskeleton>spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
| Tissue specificity | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | Involved in stress resistance and actin organization. |
Images
Western Blot analysis of 22RV1 cells using HSP27 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human stomach cancer tissue using HSP27 Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse kidney tissue using HSP27 Polyclonal Antibody at dilution of 1:200.
