Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)
丙二醛(MDA)比色法测试盒(TBA法)
| 规格： | / 50 assays(48 samples) / 100 assays(96 samples) |
| 价格： | / ￥280 / ￥500 |
货号：E-BC-K025-S
检测仪器及波长: 紫外-可见分光光度计（532 nm）
样本类型: 血清（浆）;动物组织
检测原理
过氧化脂质降解产物中的丙二醛（MDA）在高温及酸性环境下可与硫代巴比妥酸（TBA）反应产生红棕色产物3,5,5’-三甲基恶唑2,4-二酮（三甲川），该物质在532nm处有最大吸收峰。
关键信息
| 指标简称 | MDA |
| 样本类型 | 血清（浆）;动物组织 |
| 灵敏度 | 0.38 nmol/mL |
| 检测范围 | 0.38-133.33 nmol/mL |
| 精密度 | 批间差：8%批内差：4.9% |
| 检测仪器及波长 | 分光光度计 |
| 特殊仪器 | 100℃恒温水浴锅 |
| 自备试剂 | 冰乙酸、无水乙醇 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围（0.38-133.33 nmol/mL），请参考下表稀释:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
10%大鼠心匀浆 |
不稀释 |
|
人血浆 |
不稀释 |
10%大鼠肝匀浆 |
不稀释 |
|
大鼠血清 |
不稀释 |
10%大鼠脾匀浆 |
不稀释 |
|
大鼠血浆 |
不稀释 |
10%大鼠肺匀浆 |
不稀释 |
|
小鼠血清 |
不稀释 |
10%大鼠肾匀浆 |
不稀释 |
|
小鼠血浆 |
不稀释 |
10%大鼠脑匀浆 |
不稀释 |
注：稀释液为生理盐水或PBS（0.01 M，pH 7.4）。
相关产品
| 货号 | 产品名称 |
|---|---|
| E-BC-K031-S | 过氧化氢酶(CAT) 比色法测试盒 |
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