p16 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-40264
宿主: Rabbit
反应性: H,R
应用: IHC,IF
Overview
| Synonyms | CCM2,CDK4 inhibitor p16 INK4,CDK4I,CDKN2,CDKN2A,Cell cycle negative regulator beta,CMM2,Cyclin dependent kinase 4 inhibitor A,Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4),Cyclin Dependent Kinase Inhibitor 2A,p16抗体 |
| Swissprot | Q9R0Z3 |
| Source | Rabbit |
| Reactivity | Human,Rat |
| Immunogen | Recombinant Rat Cyclin-dependent kinase inhibitor 2A protein |
| Application | IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | IHC,,1:50-1:200;IF,,1:100-1:200; |
| Concentration | 0.82mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm, Nucleus |
| Tissue specificity | Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
| Background | This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. |
Images
Immunohistochemistry of paraffinembedded Rat liver using p16 Polyclonal Antibody at dilution of 1:100
Immunohistochemistry of paraffinembedded Human ovarian using p16 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of PC-3 cells using p16 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of PC-3 cells using p16 Polyclonal Antibody at dilution of 1:100
