PCNA Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-18205
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC,ELISA
Overview
| Synonyms | ATLD2,cb16,Cyclin,DNA polymerase delta auxiliary protein,etID36690.10,fa28e03,fb36g03,HGCN8729,MGC8367,Mutagen-sensitive 209 protein,OTTHUMP00000030189,OTTHUMP00000030190,PCNA,Pcna/cyclin,PCNA,PCNAR,Polymerase delta accessory protein,Proliferating cell nuclear antigen,wu:fa28e03,wu:fb36g03,PCNA抗体 |
| Swissprot | P12004 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Fusion protein of human PCNA |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213),ELISA |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:25-1:100;ELISA,,1:5000-1:10000; |
| Concentration | 0.7mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. |
Images
Western blot analysis of 293T and HEPG2 cell lysates using PCNA Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human prost at e cancer tissue using PCNA Polyclonal Antibody at dilution of 1:35(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue using PCNA Polyclonal Antibody at dilution of 1:35(×200)
