PCNA Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70285
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | ATLD2,cb16,Cyclin,DNA polymerase delta auxiliary protein,etID36690.10,fa28e03,fb36g03,HGCN8729,MGC8367,Mutagen-sensitive 209 protein,OTTHUMP00000030189,OTTHUMP00000030190,PCNA,Pcna/cyclin,PCNA,PCNAR,Polymerase delta accessory protein,Proliferating cell nuclear antigen,wu:fa28e03,wu:fb36g03,Proliferating Cell Nuclear Antigen抗体 |
| Swissprot | Q12004,P17918,P04961 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein corresponding to Mouse PCNA |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:500-1:1000; |
| Concentration | 470 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus |
| Tissue specificity | Highest expression level in thymus |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Proliferating Cell Nuclear Antigen,commonly known as PCNA,is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. |
Images
Western Blot analysis of various samples using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded Rat lung using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:1000.
