Urea (BUN) Colorimetric Assay Kit (Urease Method)
尿素(BUN)比色法测试盒(脲酶法)
| 规格： | / 50 assays(24 samples) / 100 assays(48 samples) |
| 价格： | / ￥180 / ￥280 |
货号：E-BC-K183-S
检测仪器及波长: 紫外-可见光分光光度计（580 nm）
样本类型: 血清（浆）;尿液;唾液;乳汁
检测原理
尿素在脲酶的作用下分解产生铵离子和二氧化碳，铵离子在碱性介质中与酚衍生物生成绿色的物质，该物质的生成量与尿素含量呈正比。
关键信息
| 指标简称 | BUN,UN |
| 样本类型 | 血清（浆）;尿液;唾液;乳汁 |
| 灵敏度 | 0.114 mmol/L |
| 检测范围 | 0.114-30 mmol/L |
| 精密度 | 批间差：4.7%批内差：4.6% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 3个月 |
样本稀释倍数
在正式检测前，需选择2-3例预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围（0.114-30 mmol/L），请参考下表稀释：
注：稀释液为PBS（0.01 M，pH 7.4）或生理盐水。
|
尿素浓度（mmol/L） |
样本与稀释液的体积比 |
稀释倍数 |
|
<30 |
不稀释 |
1 |
|
30-300 |
1:9 |
10 |
|
300-3000 |
1:99 |
100 |
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