Vimentin Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70081
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | CTRCT30,Epididymis luminal protein 113,FLJ36605,HEL113,VIM,VIME,Vimentin,Vimentin抗体 |
| Swissprot | P08670,P20152,P31000 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse vimentin |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:200-1:800; |
| Concentration | 1.1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm |
| Tissue specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.Highest expression level in dorsal root ganglion. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Vimentin,also named as VIM,belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092),suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. |
Images
Western Blot analysis of various samples using Vimentin Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Vimentin Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded Human colon using Vimentin Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Vimentin Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded Human Kidney using Vimentin Polyclonal Antibody(Elabscience® Product Detected by Lifespan).
客户发表文献
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