Catalase (CAT) Activity Assay Kit
过氧化氢酶(CAT) 比色法测试盒
| 规格： | / 48T(16 samples) / 96T(40 samples) |
| 价格： | / ￥480 / ￥860 |
货号：E-BC-K031-M
检测仪器及波长: 酶标仪（400-410 nm，最佳405nm）
样本类型: 血清;血浆;培养细胞;培养液及动植物组织样本
检测原理
过氧化氢酶催化H2O2分解为氧和水，钼酸铵能够迅速中止CAT分解H2O2的反应，而剩余的H2O2与钼酸铵作用产生一种淡黄色的络合物，在波长405 nm处测定其生成量，可计算出CAT的活力。
关键信息
| 指标简称 | CAT |
| 样本类型 | 血清;血浆;培养细胞;培养液及动植物组织样本 |
| 灵敏度 | 1.12 U/mL |
| 检测范围 | 1.12 -150 U/mL |
| 精密度 | 批间差：7.7%批内差：3.9% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
本试剂盒的检测范围在1.12 -150 U/mL，测试前选择2-3个预期差异大的样本进行预实验以确定最佳取样浓度。不同样本稀释比例范围如下表（仅供参考）：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
小鼠血清 |
不稀释 |
|
293T细胞上清 |
不稀释 |
10%绿萝匀浆 |
1-2 |
|
10%大鼠心匀浆 |
50-100 |
10%大鼠肺匀浆 |
50-100 |
|
10%大鼠肝匀浆 |
100-200 |
10%大鼠肾匀浆 |
50-100 |
|
10%大鼠脾匀浆 |
50-100 |
10%大鼠脑匀浆 |
20-50 |
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
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