Human IL-6(Interleukin 6) ELISA Kit

产品货号:E-EL-H0102c

规格: / 96T / 48T
价格: / ¥2400 / ¥1650

货号:E-EL-H0102c

货期:3个工作日+顺丰周期

反应性:

检测范围: 7.81~500 pg/mL

灵敏度: 4.69 pg/mL

  • 详情
  • 文献(29)
  • 用途

    该试剂盒用于体外定量检测人Serum, plasma and other biological fluids中天然及部分重组IL-6浓度

    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上,实验时样品或标准品中的人IL-6会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。

    反应类型 夹心法
    规格 96T
    反应时间 3.5h
    反应性
    检测方法 Colormetric
    检测范围 7.81—500 pg/mL
    灵敏度 4.69 pg/mL
    样本体积 100μL
    样本类型 Serum, plasma and other biological fluids

    特异性

    可检测样本中的人IL-6,且与其它相关蛋白无明显交叉反应。

    重复性

    板内,板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (pg/mL) O.D Average Corrected
    500 2.463
    2.487
    2.475 2.407
    250 1.612
    1.636
    1.624 1.556
    125 1.012
    0.978
    0.995 0.927
    62.5 0.513
    0.533
    0.523 0.455
    31.25 0.257
    0.245
    0.251 0.183
    15.63 0.174
    0.17
    0.172 0.104
    7.81 0.119
    0.123
    0.121 0.053
    0 0.067
    0.069
    0.068 --

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

    Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (pg/mL)
    25.30 67.60 210.10 24.20 66.50 197.40
    Standard
    deviation
    1.70 2.80 7.80 1.60 3.40 6.70
    C V (%) 6.72 4.14 3.71 6.61 5.11 3.39

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 97-110 102
    EDTA plasma (n=5) 92-108 99
    Cell culture media (n=5) 95-109 101

    线性

    分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

    Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
    1:2 Range (%) 100-111 89-103 88-101
    Average (%) 105 97 93
    1:4 Range (%) 97-108 83-96 94-109
    Average (%) 102 90 100
    1:8 Range (%) 94-109 83-97 95-110
    Average (%) 101 89 102
    1:16 Range (%) 99-112 84-96 93-105
    Average (%) 106 88 99

    试剂盒组成及保存

    未拆封的试剂盒可在4℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2 支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 4℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 4℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 4℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1. 酶标仪(450nm波长滤光片)
    • 2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3. 37℃恒温箱,双蒸水或去离子水
    • 4. 吸水纸

    操作步骤

    1.对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟
    2.弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟
    3.弃掉板内液体,洗板3次
    4.每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次
    5.每孔加入90μL底物溶液,37℃孵育15分钟
    6.每孔加入50μL终止液
    7.立即在450nm波长下读数,处理数据

    客户发表文献

    1. 文献: Li G Y , Liao Y , Hu J J , et al. Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure[J]. Environmental Pollution, 2019, 252: 1216-1224.
      样品类型: Cell culture supernatant
    2. 文献: Ruan G, Xu J, Wang K, et al. Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis[J]. Osteoarthritis and cartilage, 2018.
      样品类型: Serum
    3. 文献: Zhou P, Liu H, Wu Y, et al. Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response[J]. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research, 2018, 24: 4379.
      样品类型: Serum
    4. 文献: Wang F, Li X, Huang L, et al. High-Mobility Group Protein Box 1 is Upregulated in Children with Henoch-Schonlein Purpura[J]. Pediatric Allergy, Immunology, and Pulmonology, 2018, 31(2): 66-72.
      样品类型: Serum
    5. 文献: Akash M S H, Rehman K, Liaqat A, et al. Biochemical investigation of gender-specific association between insulin resistance and inflammatory biomarkers in types 2 diabetic patients[J]. Biomedicine & Pharmacotherapy, 2018, 106: 285-291.
      样品类型: Serum
    6. 文献: Kruger M J, Conradie R, Conradie M, et al. ADSC-conditioned media elicit an ex vivo anti-inflammatory macrophage response[J]. Journal of molecular endocrinology, 2018: JME-18-0078.
      样品类型: Serum, Cell culture medium
    7. 文献: Lu A Q, Wang Z R, Wang S C. Role Of miR-589-3p In Human Lumbar Disc Degeneration And Its Potential Mechanism[J]. Experimental and Therapeutic Medicine, 2018, 15: 1616-1621.
      样品类型: Cell culture supernatant
    8. 文献: Pan Y Y, Zhou F H, Song Z H, et al. Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation[J]. Biomedicine & Pharmacotherapy, 2018, 97: 1694-1700.
      样品类型: Cell culture supernatant,Serum
    9. 文献: Jiang Z, Wang W H, Guo C C. Tetrahydroxy Stilbene Glucoside Ameliorates H2O2-Induced Human Brain Microvascular Endothelial Cell Dysfunction In Vitro By Inhibiting Oxidative Stress And Inflammatory Responses[J]. Molecular Medicine Reports, 2017, 16(4): 5219-5224.
      样品类型: Cell culture supernatant
    10. 文献: Liu Q, Wu J B, Song J Q, et al. Particulate Matter 2.5 Regulates Lipid Synthesis And Inflammatory Cytokine Production In Human SZ95 Sebocytes[J]. International Journal of Molecular Medicine, 2017, 40(4): 1029-1036.
      样品类型: Cell culture supernatant
    11. 文献: Wang N N, Guo J, Liu F D, et al. Depot-Specific Inflammation With Decreased Expression Of ATM2 In White Adipose Tissues Induced By High-Margarine/Lard Intake[J]. Plos One, 2017.
      样品类型: Cell culture supernatant
    12. 文献: Li Z, Tang Y, Tang N, et al. High Anti-Human Cytomegalovirus Antibody Levels Are Associated With The Progression Of Essential Hypertension And Target Organ Damage In Han Chinese Population[J]. Plos One, 2017.
      样品类型: Serum
    13. 文献: Cao Z, Chen L. Inhibition Of miR27a Suppresses The Inflammatory Response Via The p38/MAPK Pathway In Intervertebral Disc Cells[J]. Experimental and Therapeutic Medicine, 2017, 14(5): 4572-4578.
      样品类型: Cell culture supernatant
    14. 文献: Xu H, Sun Q, Lu L, et al. MicroRNA-218 Acts By Repressing TNFR1-Mediated Activation Of NF-κB, Which Is Involved In MUC5AC Hyper-Production And Inflammation In Smoking-Induced Bronchiolitis Of COPD[J]. Toxicology Letters, 2017, 280: 171-180.
      样品类型: Serum,Cell culture supernatant
    15. 文献: Lei C T, Su H, Ye C, et al. The Classic Signalling And Trans-signalling Of Interleukin-6 Are Both Injurious In Podocyte Under High Glucose Exposure[J]. Journal of Cellular and Molecular Medicine, 2017.
      样品类型: Serum
    16. 文献: Chen W H, Luo G F, Lei Q, et al. Overcoming The Heat Endurance Of Tumor Cells By Interfering The Anaerobic Glycolysis Metabolism For Improved Photothermal Therapy[J]. Acs Nano, 2017, 11(2): 1419-1431.
      样品类型: Cell culture supernatant
    17. 文献: He W, Ren Y, Wang X, et al. C Reactive Protein And Enzymatically Modified LDL Cooperatively Promote Dendritic Cell-Mediated T Cell Activation[J]. Cardiovascular Pathology, 2017, 29: 1-6.
      样品类型: Cell culture supernatant
    18. 文献: Li L, Zhang J, Zhao M, et al. Applied Value Of Monitoring Serum Hepcidin In Differential Diagnosis Of Infection Versus Tumor Fevers[J]. Journal of Huazhong University of Science and Technology-Medical Sciences, 2017, 37(2): 253-256.
      样品类型: Plasma
    19. 文献: Park K S, Kim S H, Das A, et al. TLR3-/4-Priming Differentially Promotes Ca2+Signaling And Cytokine Expression And Ca2+-Dependently Augments Cytokine Release In hMSCs[J]. Scientific Reports, 2016.
      样品类型: Cell culture supernatant
    20. 文献: Zhao J, Liang Y, Song F, et al. TSG Attenuates LPC-Induced Endothelial Cells Inflammatory Damage Through Notch Signaling Inhibition[J]. IUBMB Life, 2016, 68(1): 37-50.
      样品类型: Cell culture supernatant
    21. 文献: Li W, Dong H, Huang Y, et al. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients[J]. Plos Neglected Tropical Diseases, 2016.
      样品类型: Serum,Cell culture supernatant
    22. 文献: Zhao Y, Jiang C, He J, et al. Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages And Promote Beneficial Antiatherosclerotic Mechanisms[J]. Bioconjugate Chemistry, 2016.
      样品类型: Cell culture supernatant
    23. 文献: Tian P, Li B, He C, et al. Antidiabetic (Type 2) Effects Of Lactobacillus G15 And Q14 In Rats Through Regulation Of Intestinal Permeability And Microbiota[J]. Food & Function, 2016.
      样品类型: Plasma,Serum
    24. 文献: Cheng P, Hua W, Zhang W J, et al. Inhibitory Effect Of miR-125b On Hepatitis C Virus Core Protein-Induced TLR2/MyD88 Signaling In THP-1 Macrophages[J]. World Journal of Gastroenterology, 2016, 22(17): 4354-4361.
      样品类型: Cell culture supernatant
    25. 文献: Hao S Y, Ji J J, Zhao H T,et al. Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation Through Both ROS Scavenging And Inhibition Of Cholesterol Influx In Raw264.7 Cells[J]. Molecules, 2015, 20(12): 21287-21297.
      样品类型: Cell lysate
    26. 文献: Veena V K, Popavath R N, Kennedy K, et al. In Vitro Antiproliferative, Pro-Apoptotic, Antimetastatic And Anti-Inflammatory Potential Of 2, 4-Diacteylphloroglucinol (DAPG) By Pseudomonas Aeruginosa Strain FP10[J]. Apoptosis, 2015, 20(10): 1281-1295.
      样品类型: Cell culture supernatant
    27. 文献: Tang J N, Shen L, Liu C L, et al. Plasma Levels Of C1q/TNF-Related Protein 1 And Interleukin 6 In Patients With Acute Coronary Syndrome Or Stable Angina Pectoris[J]. American Journal of The Medical Sciences, 2015, 349(2): 130-136.
      样品类型: Plasma
    28. 文献: Liu J, Lu C Y, Zhou H, et al. Flexible Gold Electrode Array For Multiplexed Immunoelectrochemical Measurement Of Three Protein Biomarkers For Prostate Cancer[J]. ACS Applied Materials & Interfaces, 2014, 6(22): 20137-20143.
    29. 文献: Liao Y, Liu P, Guo F, et al. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human[J]. Plos One, 2013.
      样品类型: Plasma
    如有任何问题,请拨打电话 027-87879180 或填写 在线留言 我们会第一时间联系您。